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題名 | Agronomic Characteristics of Various Cultivars of Common Bean (Phaseolus Vulgaris L.) and Analysis of Their Seed Compositions=不同品系矮性菜豆農藝性狀之探討及其豆仁化學組成之分析 |
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作者 | 胡懋麟; 黃天成; 邱義源; | 書刊名 | 中國農業化學會誌 |
卷期 | 35:1 1997.02[民86.02] |
頁次 | 頁9-17 |
分類號 | 435.291 |
關鍵詞 | 矮性菜豆; 農藝性狀; 組成分析; 胺基酸組成; 醣類; 電泳分析; Phaseolus vulgaris L.; Agronomic characteristics; Compositional analysis; Amino acid composition; Carbohydrate; SDS-PAGE; |
語文 | 英文(English) |
英文摘要 | 以北港在來、白長鶉、A 463、ICA 21148 及 PI 201494 等五種不同品系矮性菜 豆進行農藝性狀探討及化學組成分析。以冬季裡作栽種時,平均生長期、株高、每株豆莢數 、每莢種仁數、平均百粒重及每公頃產量分別為 79~93 天、 26.5~32.0cm、5.8~10.1 莢、 3.4~4.8 粒、33.3~52.6g 及 1280~1530kg。 其種仁之平均水分、粗蛋白、粗脂肪、醣類及 灰分含量分別為 8.57~11.62%、18.56~20.06%、4.82~9.51%、55.04~62.89% 及 3.71~4.60% 。總胺基酸組成在品系間之差異不甚明顯,其蛋白質皆含相當高比例之組胺酸、異白胺酸、 纈胺酸、離胺酸、苯丙胺酸、酪胺酸及蘇胺酸等。至於寡醣、蔗糖及游離胺基酸組成,則因 品系之不同而有不同程度的差異。當以磷酸鹽緩衝液 (0.2M,pH7.9) 抽取可溶性蛋白質並以 SDS-PAGE 分析時, 五種品系蛋白質圖譜間之定性差異不明顯, 皆含分子量為 65,000 及 45,000 Daltons 之主要蛋白質組成單位,僅 ICA 21148 另含分子量約 36,000 Daltons 類 似 arcelin (一種抗昆蟲蛋白 ) 之蛋白質組成單位。 common bean proteins in comparison to some other legume proteins. However, sucrose, oligosaccharide and freee amino acid contents varied significantly among cultivars. When soluble proteins were extracted with phosphate buffer (0.2M, pH7.9) and subjected to SDS-PAGE analysis, the protein patterns varied quantitatively rather than qualitatively, and two major subunits with M.W. ca. 65,000 and 45,000 Daltons were observed. An additional arcelin-like subunit with M.W. ca. 36,000 Daltons was observed in the extracted proteins of ICA 21148. |
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