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題 名 | 天王補心丹對大白鼠單向被動迴避學習反應之影響=Effects of Tien-Wang-Pu-Hsin-Dang on the One-Trial Inhibitory Step-Through Passive Avoidance in Rats |
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作 者 | 謝明村; | 書刊名 | 中醫藥年報 |
卷 期 | 15:2 1997.05[民86.05] |
頁 次 | 頁450-475 |
分類號 | 414.5 |
關鍵詞 | 天王補心丹; 學習記憶; Tien-wang-pu-hsin-dang; Learning and memory; Scopolamine; Cycloheximide; |
語 文 | 中文(Chinese) |
中文摘要 | 天王補心丹主治 「思慮過度,心血不足,怔忡健忘,心口多汗,大便或秘或溏, 口舌生瘡等證」,方由人參、元參、丹參、茯苓、酸棗仁、遠志、柏子仁、天門冬、麥門冬 、生地黃、桔梗、當歸及五味子等十三味藥所組成; 方中以生地、玄參、麥冬及天冬滋陰制 火為君:人參及當歸補益氣血為臣:棗仁、柏子仁、丹參、茯苓、遠志、五味子養血斂心、 鎮心安神為佐,桔梗載藥人心為使。,心者,君子之官也,神明出焉,思慮過度,耗其心血 ,則神明傷而成心勞,故怔忡健忘。因此本研究將天王補心湯分為君藥組 (生地、玄參、麥 冬及天冬 )、臣藥組 (人參及當歸 )、佐藥組 (棗仁、柏子仁、丹參、茯苓、遠志、五味子 ) 、 使藥組 (桔梗 ) 及全方組等五組, 擬以單向被動迴避反應 (one-trial passive avoidance) 及自發運動量來探討天王補心丹對 scopolamine 及 cycloheximide 所誘發之 學習記憶障礙之作用及天王補心丹方劑組成與主治間之關連性。一次給藥之天王補心丹各組 ,對 scopolamine 誘發之學習獲得障礙及 cycloheximide 誘發之記憶鞏固障礙,僅天王補 心丹全方組之水粗抽組及 95% 酒精粗抽組其改善作用, 且以水粗抽組較佳; 至於對大鼠自 發運動量, 天工補心丹全力組之水粗抽組及 95% 酒精粗抽組具降低大鼠自發運動量之作用 , 但對電痛閾值則各組均不影響。 一週連續給藥之天王補心丹各組在大劑量時, 對 scopolamine 誘發之學習獲得障礙及 cycloheximide 誘發之記憶鞏固障礙均具改善作用, 其中以天王補心丹全方組之水粗抽組較為明顯; 其次在自發運動量的試驗,水粗抽組具增加 大鼠自發運動量之作用, 但對電痛閾值則不影響。 最後, 全方組之水粗抽組改善 cycloheximide 誘發記憶鞏固障礙之作用,可被 scopolamine 及 8 一 OHDPAT 所拮抗,為 ritanserin 所增強。綜合研究結果,顯示天王補心丹全方組之水粗抽組及 95% 酒精粗抽組 ,在一次給藥後,具改善學習記憶作用,且以水粗抽者較佳,而其改善學習記憶部份因鎮靜 之作用所致「一週連續給藥後,各組在大劑量下對於學習獲得及記憶鞏固障礙均有明顯之改 善作用。而其改善學習記憶障礙之作用機轉可能與增強中樞 cholinergic system 之活性及 protein synthesis、降低 serotonergic system 之活性有關。 |
英文摘要 | In this study, we divided the Tien-Wang-Pu-Hsin-Dang (abbr. as TWP) into five groups: (1) Chun was consisted of Rehmanniae Radix, Schrophulariae Radix, Ophiopogonis Tuber and Asparagi Radix. (2) Chen was consisted of Ginseng Radix and Angelicae Sinensis Radix. (3) Tso was consisted of zizyphi Semen, Thujae Orientalis Semen, Salivae Miltiorrhizae Radix, Hoelen, Polygalae Radix and Schizandrae Fructus. (4) Shih was consisted of Platycodi Radix. (5) TWP was consisted of the above four groups. We attempted to investigate the effect of five groups on scopolamine-induced and cycloheximide-induced amnesia in passive avoidance task and locomotor activity in rats. After acute treatment, only TWP extracted with water or 95 % ethanol could improved the scopolamine-induced acquisition impairment and cycloheximide- induced consolidation deficit, and the former was better than the latter. After one-week consecutive treatment, five groups could improved the scopolamine-induced acquisition impairment and cycloheximide- induced consolidation deficit, and TWP extracted with water was better than the others. Whereas, TWP extracted with water decreased the locomotor activity after acute treatment and increased the locomotor activity after one-week consecutive treatment in rats. But five groups did not change the threshold of vocalization with electric foot-shock in rats. Furthermore, the memory improvement of TWP on the cycloheximide-induced storage impairment was antagonized by scopolamine and 8-OHDPAT, and was potentiated by ritanserin. These results are shown that TWP produce memory improvement in acute treatment, partially due to possess sedative effect. The learning and memory improvement effect of TWP after one- week consecutive treatment may be due to the increase in cholinergic activity and protein synthesis, and the decrease in serotonergic activity. |
本系統中英文摘要資訊取自各篇刊載內容。