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頁籤選單縮合
題名 | 鰻魚愛德華氏菌、產氣單胞菌、滑走細菌混合菌苗之研製及田間應用試驗=Development and Field Application of Edwardsiella Tarda、Aeromonas Hydrophila and Flexibacter Columnaris Combined Bacterin in Eels |
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作者 | 柯浩然; 陳清; 呂清泉; 賴俊雄; 郭乃維; 詹益波; 蕭澤民; 陳秀男; 王進添; 張鴻猷; Ko, H. J.; Chen, Ching; Lu, C. C.; Lai, J. S.; Guo, Nae Wei; Chan, I. P.; Shau, C. M.; Chen, S. N.; Wang, J. T.; Chang, H. Y.; |
期刊 | 臺灣省家畜衛生試驗所研究報告 |
出版日期 | 19970000 |
卷期 | 33 1997[民86.] |
頁次 | 頁71-77 |
分類號 | 439.5 |
語文 | chi |
關鍵詞 | 鰻魚; 愛德華氏菌; 產氣單胞菌; 滑走細菌; 混合菌苗; Eels; Edwardsiella tarda; Aeromonas hydrophila; Flexibacter columnaris; Combination bacterin; |
中文摘要 | 研製之愛德華氏菌、產氣單胞菌及滑走細菌不活化混合菌苗分別以肌肉注射免疫 方式( 1.0 mL/ 尾)與混於餌料口服免疫之方式( 6.0 mL/ 尾; 連續 2 日)免疫鰻魚, 免疫後分別在第 2 週(肌肉注射免疫組)及第 4 週(口服免疫組)以愛德華氏菌、產氣單 胞菌、滑走細菌及三者混合菌液之不同濃度攻擊結果。得知其免疫力價,在本所試驗鰻魚池 肌肉注射免疫組防禦指數分別 Log 10、Log 10、Log 10 及 Log10;口服免疫組防禦指數為 Log 10、 Log 10、Log 10 及 Log 10。 在臺西水試分所試驗鰻魚防禦指數分別為 Log 10、Log 10、 Log 10 及 Log 10。 由以上試驗結果顯示所研製之不活化混合菌苗雖在防禦力價有高低差異,但整體而言免疫後 鰻魚均可產生良好防禦效果,對愛德華氏病、赤鰭病與腐鰓病具有預防效益。 |
英文摘要 | An inactivated bacterin consisting of Edwardsiella tarda, Aeromonas hydrophila and Flexibacter columnaris was developed. Immunization test using intramuscular injection (IM) and orally with fish meal were carried out in eels to evaluate the combined bacterins. Each eel in the IM group was injected with 1.0 mL of bacterin; the eels in the orally vaccination with meal group were given 6.0 mL per eel per day for two consecutive days. Two weeks and four weeks after immunization, respectively, for the IM group and the orally group, the eels were challenged with Edwardsiella tarda, Aeromonas hydrophila, Flexibacter columnaris and it's combined suspension on pathogens. respectively. The protection indexes (PI) obtained in the laboratory were log10,log10,log10 and log10 for the IM group and log10,log10,log10 and log10 for the orally group, respectively. Using the same pathogens in the laboratory, the test were also conducted in Taishi branch of the TFRI, The PI of by using orally vaccination with meal were log10,log10,log10 and log10,respectively. These experimental results showed that there are variation in the protection indexes after challenge. As a whole, however, the combined bacterin achieved good immunization results in eels against the Edwardsiellosis, red fin disease,and columnaris disease. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。