查詢結果分析
相關文獻
- Application of Polymerase Chain Reaction for Rapid Diagnosis of Tuberculous Meningitis
- Clinical Application of Polymerase Chain Reaction Assay in Early Diagnosis of Tuberculous Meningitis
- Detection and Identification of Mycobacterium Tuberculosis by Nested PCR Assays in Cerebrospinal Fluid Samples from Patients with Suspected Tuberculous Meningitis
- Diagnosis of Tuberculous Meningitis by a Nested Polymerase Chain Reaction
- Drug Resistance Patterns of Tuberculosis in Taiwan
- Cloning and Characterization of Insulin-Like Growth Factor I cDNA from Black Seabream (Acanthopagrus Schlegeli)
- 應用反轉錄聚合酶鏈反應檢測1996年臺灣流行之牛流行熱
- 應用聚合酶鏈反應檢測臺灣牛隻牛白血病Proviral DNA
- 臺灣母山羊流產症之病因探討與疫學研究
- 利用反轉錄聚合酶鏈反應技術在新城雞病病毒臺灣分離株之檢測
頁籤選單縮合
題 名 | Application of Polymerase Chain Reaction for Rapid Diagnosis of Tuberculous Meningitis=應用聚合酶鏈反應快速診斷結核性腦膜炎 |
---|---|
作 者 | 袁瑞昱; 林秀偉; 葉健全; | 書刊名 | 北醫學報 |
卷 期 | 25:1 1996.06[民85.06] |
頁 次 | 頁1-6 |
分類號 | 415.9313 |
關鍵詞 | 結核性腦膜炎; 聚合酶鏈反應; 結核桿菌; 去氧核糖核酸; Polymerase chain reaction; Tuberculous meningitis; Mycobacterium tuberculosis; DNA; |
語 文 | 英文(English) |
中文摘要 | 我們應用聚合□鏈反應(polymerase chain reaction;PCR)放大去氧核糖核酸(Deoxyribonucleic acid;DNA)的方法,偵測存在腦脊髓液(cerebrospinal fluid;CSF)裡結核桿菌整組基因(M. tuberculosis genome)中的片斷DNA,以早期正確的診斷結核性腦膜炎(tuberculous meningitis;TBM)。利用PCR將TBM病人CSF檢體中具多重複製(multiple copies)且專屬結核桿菌特定嵌入排列順序(insertion sequence)IS6110中的一小段擁有123個鹽基配(base pairs;bp)的寡核甘酸(oligonucleotides)加以複製放大,再經由電泳(electrophoresis)在瓊脂凝膠(agarose gel)中將此段123 bp的PCR複製產物分離出來,用ethidium bromide染色,經過紫外光透視,陽性的CSF檢體可以用肉眼辨視出一條包含有123 bp的特殊“帶”(band)。如此在兩個工作天之內,就可以得到PCR檢驗的結果。利用此PCR方法,評估五個臨床診斷為TBM病人的五個CSF檢體,有四個CSF檢體得到PCR陽性的結果。在這四個PCR陽性的CSF檢體,只有一個用結核桿菌培養的方法得到陽性。至於直接用CSF檢體抹片,抗酸性染色檢視結核桿菌的方法,卻沒有任何一個CSF檢體得到陽性。而二十六個臨床上非TBM病人的CSF檢體全部都沒有得到PCR陽性。 這個成果可以表示,PCR檢查比起傳統的細菌學方法使用在探查CSF中是否存有結核桿菌上更具敏感度而且有一定的特異性,可以應用於臨床上早期確立TBM的診斷。 |
英文摘要 | We applied a rapid diagnostic method based on the DNA amplification by polymerase chain reaction (PCR) to detect Mycobacterium tuberculosis genome in cerebrospinal fluid (CSF). The target DNA sequences to be amplified were a 123 base pairs (bp) fragment of the IS6110 insertion elements which occur in multiple copies in the mycobacterial genome. PCR amplification products were elec-trophoresed and positive results were identified only by the inspection of a band in 123 bp region of a ethidium bromide -stained agarose gel. Using this PCR assay, we can detect M. tuberculossis genome in CSF within two working days. Of 5 CSF smaples from 5 patients with tuberculous meningitis (TBM) clinically, the PCR was positive in 4 samples, whereas M. tuberculosis cultures were positive in only one sample. None was positive for the acid-fast bacillus (AFB) by direct smear. The PCR was negative in all the 26 CSF smaples obtained from the non-TBM control group. The results revealed that the PCR assay is specific and more sensitive than conventional bacteriologic method for the rapid diagnosis of TBM. |
本系統中英文摘要資訊取自各篇刊載內容。