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題名 | 應用聚合酶鏈鎖反應檢測鴨B型肝炎病毒= |
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作者 | 周世認; 劉世東; 沈永紹; |
期刊 | 國立臺灣大學農學院研究報告 |
出版日期 | 19950300 |
卷期 | 35:1 1995.03[民84.03] |
頁次 | 頁92-106 |
分類號 | 437.246 |
語文 | chi |
關鍵詞 | 鴨B型肝炎病毒; 聚合酶鏈鎖反應; Duck hepatitis B virus; DHBV; Polymerase chain reaction; PCR; |
中文摘要 | 應用聚合�t鏈鎖反應(polymerase chain reaction,PCR)檢測162隻台灣產蛋 雌性菜鴨與20隻雄性菜鴨之血清及精液,以及20個菜鴨蛋之蛋黃及蛋白中之鴨B 型肝炎病毒(duck hepatitis B virus,DHBV),結果DHBV陽性率分別為:產蛋雌性菜 鴨之血清為23.46(38/162)、雄性菜鴨之血清及精液各為10(2/20)、菜鴨蛋之蛋黃為 0(0/20)而蛋白為10(2/20),與點狀雜交法(dot blothybridization)之結果相同。 PCR之產物再以1對巢狀引子 (nested primer)用PCR增幅後之結果,DHBV陽性率分 別為產蛋雌性菜鴨之血清為57.41(93/162)、雄性菜鴨之血清及精液均為30(6/20)、 菜鴨蛋之蛋黃為30(6/20)、蛋白為30(6/20),比第1次PCR之結果產蛋雌性菜鴨之血 清增加33.95(55/162)、雄性菜鴨之血清及精液各增加20(4/20)、菜鴨蛋之蛋黃增加 30(6/20)而蛋白增加20(4/20)。PCR之優點為快速,可在1天內完成且非常敏感,及 不需使用放射性標識試劑 |
英文摘要 | Duck hepatitis B virus (DHBV) was assayed by using thepolymerase chain reaction (PCR) in the serum of 162 female Chinesecommon domestic ducks and the serum and the semen of 20 maleducks, and the yolk and albumen of 20 eggs as well. Consequently,the DHBV positive rate of each group was as follows: the serum ofthe female Chinese common domestic ducks, 24.36 (38/162); maleducks, 10 (2/20); the yolk of the eggs, 0 (0/20); and the albumen,10 (2/20). The result was same as by the dot-blot hybridizationtechnique. Then the products of PCR were amplified again by PCR with apair of nested primer. The DHBV positive rate of each group was57.41 (93/162) in the serum of female ducks, 30 (6/20) in the serumand the semen of male ducks, 30 (6/20) in the yolk and 30 (6/20)in the albumen of eggs. Compared with the first PCR, the result ofthe second PCR increased by 33.95 (55/162) in the serum of female ducks, 20 (4/20) in the serum and the semen of male ducks, 30(6/20) in the yolk and , 20 (4/20) in the albumen of eggs. The advantages of PCR are that the assay can be completed in I day, being very sensitive, and dose not require the use of radiolabeled reagents. |
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