查詢結果分析
相關文獻
- Use of Southern Hybridization to Detect the Haemophilus Influenzae β-lactamase Gene
- Mitochondrial Myopathy with Predominant Respiratory Dysfunction in a Patient with A3243G Mutation in the Mitochondrial tRNA Gene
- Polymorphisms of Twelve Short Tandem Repeat Loci in a Taiwanese Population and Their Application in Parentage Testing
- 酚類資化酵母菌Candiada tropicalis酚類羥化酶基因的選殖
- Genetic Analysis of the Insulin Receptor Gene in Chinese Patients with Extreme Insulin Resistance
- Germline RET Proto-Oncogene Mutations in Two Taiwanese Families with Multiple Endocrine Neoplasia Type 2A
- RET Protooncogene Mutations in Patients with Apparently Sporadic Medullary Thyroid Carcinoma
- HLA DQA1 Genotyping of Chinese in Taiwan by PCR-RFLP
- Machining Parameters Selection for Stock Removal Turning in Process Plans Using a Float Encoding Genetic Algorithm
- 傳染性支氣管炎臺灣分離株S1基因之定序及分析
頁籤選單縮合
題名 | Use of Southern Hybridization to Detect the Haemophilus Influenzae β-lactamase Gene=以南方墨點雜交法偵測流行性感冒嗜血桿菌的β-Lactamase基因 |
---|---|
作者 | 林秀真; 王志堅; 于靜梅; 朱夢麟; Lin, Hsiu-chen; Wang, Chih-chien; Yu, Cheing-mei; Chu, Mong-ling; |
期刊 | 中華民國感染症醫學會雜誌 |
出版日期 | 19970600 |
卷期 | 8:1 1997.06[民86.06] |
頁次 | 頁16-20 |
分類號 | 414.83 |
語文 | eng |
關鍵詞 | 南方墨點雜交法; 流行性感冒嗜血桿菌; 基因; Haemophilus influenzae; β-lactamase; Southern hybridization; |
中文摘要 | 會造成流行性感冒嗜血桿菌(Haemophilus influenzae)有ampicillin抗藥性的原因絕大多數是由於產生β-lactamase,而大部份的β-lactamase是屬於TEM-1型。但自1981年以來ROB-1型的β-lactamase 逐漸在流行性感冒嗜血桿菌及其他數種會造成動物感染疾病的菌株上發現。 我們共收集了45株會產生β-lactamase的流行性感冒嗜血桿菌,用南方墨點雜交方法偵測其β-lactamase基因是屬於TEM-1型或ROB-1型。此方法是以TEM-1及ROB-1基因的DNA,以非放射性物質的digoxigenin標識當做探針,再與所有流行性感冒嗜血桿菌菌株的β-lactamase 之PCR產物做雜交反應。 此次研究結果顯示,以南方墨點雜交方法偵測流行性感冒嗜血桿菌的β-lactamase可區分其屬於TEM-1型或ROB-1型。 |
英文摘要 | The most common cause of ampicillin resistance in Haemophilus influenzae is due to the production of β-lactamase. In most strains, the β-lactamase produced is TEM-1. Since 1981, ROB-1 β-lactamase appears to be responsible for ampicillin resistance in H. influenzae and several animal pathogens. Forty-five ampicillin-resistant, β-lactamase-producing clinical isolates of Haemophilus influenzae were collected. We used Southern hybridization to determine whether they produced TEM-1 or ROB-1 β-lactamase. Plasmids were obtained from a ROB-1-producing strain of H. influenzae, and a plasmid (pBR322) known to encode TEM-1. Both β-lactamase genes were labeled with non-isotope digoxigenin as probes, and then hybridized on with PCR amplified β-lactamase gene product from H. influenzae strains. The results revealed that β-lactamase-mediated ampicillin resistance type in H. influenzae could be detected by the Southern hybridization method. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。