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題名 | Studies on Infectious Laryngotracheitis in Taiwan, R.O.C.(3): Restriction Enzyme Analysis=本省雞傳染性喉頭氣管炎病毒株限制酶圖譜分析研究 |
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作者 | 謝快樂; 鄭淑文; 沈瑞鴻; 李龍湖; |
期刊 | 臺灣畜牧獸醫學會會報 |
出版日期 | 19950600 |
卷期 | 65:2 1995.06[民84.06] |
頁次 | 頁97-105 |
分類號 | 437.717 |
語文 | eng |
關鍵詞 | 傳染性喉頭氣管炎; 限制酶; Infectious laryngotracheitis; Restriction enzyme; |
中文摘要 | 為瞭解田間雞傳染性喉頭氣管炎的發生究因於減毒之弱毒株疫苗或是緊迫因子,抑是野外強毒標感染而起,擬發展出可作為臨床上快進診斷的探針,以作為病因研究之用。本試驗中之病毒株系部份來自田間發病之分離株及市面上所用之減毒疫苗株。運用限制?切割圖譜分析各株雞傳染性喉頭氣管炎病毒之去氧核醣核酸之結果顯示,各株病毒株包括田間發病株及疫苗株其去氧核醣核酸限制?切割圖譜之差異極微,僅試驗中所用PstI限制?切割圖譜上有一差異性存在。 |
英文摘要 | To establish a rapid diagnostic probe, and to determine whether clinical cases of infectious laryngotracheitis (ILT) in chickens were due to reactivation of live vaccine and the stress factors, or due to wild type ILT virus infection, DNA of ILT virus isolated from the field cases and the vaccine strains were analyzed with various endonuclease frestriction enzymes. The results of the restrction fragment patterns of ILT virus DNA, including five field isolates and two vaccine strains, showed almost no difference among each other. The only difference observed was that of the restriction fragment pattern cut by PstI restriction enzyme, in which three out of the 5field isolates showed an extra fragment at 4.0 kbp. |
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