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題名 | Acid Phosphatase in Rice Seedlings=水稻酸性磷酸酶 |
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作者 | 陳武聖; 左士嘉; 黃玉芬; 陳榮銳; Chen, W. S.; Tso, S. C.; Huang, Y. F.; Chen, Y. R.; |
期刊 | 國立臺灣大學理學院植物學系研究報告 |
出版日期 | 19920300 |
卷期 | 37:1 1992.03[民81.03] |
頁次 | 頁67-77 |
分類號 | 434.111 |
語文 | eng |
關鍵詞 | 水稻; 酸性磷酸酶; Rice seedlings; Acid phosphatase; |
中文摘要 | 水稻組織的粗抽出液在電泳分析圖譜上呈現十帶的同功酶。此粗抽出液的循步純化經硫酸銨沉澱法、Ultro膠體過濾法、DEAE-trisacryl色層法及對焦色層法等分析,取一-與p-nitorphenyl phosphate (pNPP)親和性很高的同功酶。其經FPLC法所定的分子量為130 kD,以SDS-PAGE法定出次單體的分子則分別為70 kD及62 kD。該同功酸性磷酸酶的最適pH為5.0,對pNPP的Km為0.69mM,屬熱不穩定性酵素,鎂離子及錳離子的存在可加強酵素活性,汞離子屬強抑制劑,氟化鈉則只具部分抑制性。 |
英文摘要 | Acid phosphatase (EC 3. 1. 3. 2) extracted from rice seedlings consisted of ten kinds of isozymes based on separation by polyacryamide gel electrolphoresis. One isozyme was purified through ammonium sulfate precipitation, Ultrogel gel filtration, DEAE-trisacryl chromatography and chromatofocusing. This isozyme had a high affinity to p-nitrophenyl phosphate. The molecular weight of this isozyme was around 130 kD examined with FPLC and it consisted of hetrodimer with molecular weight of 70 kD and 62 kD. The purfied isozyme had a pH optimum at 5.0 and its Km (p-nitrophyenyl phosphate) was 0.69 mM. Mg++ and Mn++ enhanced the activity, but heavy metal ions were inhibitory. NaF and Na2MoO4 also had an inhibitory effect on the isozyme. |
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