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題名 | 臺灣地區咖啡果小蠹(Hypothenemus hampei)分離之Isaria javanica CAES1鑑定與致病性測試=Identification and Pathogenicity of Isaria javanica CAES1 Isolated from the Coffee Berry Borer (Hypothenemus hampei) in Taiwan |
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作者 | 梁鈺平; 王泰權; | 書刊名 | 臺灣農業研究 |
卷期 | 69:2 2020.06[民109.06] |
頁次 | 頁132-146 |
分類號 | 434.183 |
關鍵詞 | 咖啡果小蠹; 臺灣; 生物防治; Isaria javanica; Coffee berry borer; Hypothenemus hampei; Taiwan; Biological control; |
語文 | 中文(Chinese) |
中文摘要 | 本研究自台灣咖啡田間感染真菌死亡的咖啡果小蠹(Hypothenemus hampei)上,分離到蟲生真菌CAES1,該菌株之菌落為白色至淡黃色或灰色,10-30℃均可生長,25℃菌絲生長速率最快。以光學顯微鏡檢視其無性產孢構造,其分生孢子梗分枝末端著生1-4個瓶梗,瓶梗細胞長寬約3.7-5.0-6.4 μm × 1.9-3.0-3.5μm,分生孢子串生,形狀呈橢圓形至長橢圓形,大小約為2.7-3.8-5.4 μm × 1.2-1.6-2.1 μm。以核糖體內轉錄區間(internal transcribed spacer; ITS)、translation elongation factor-1α gene(TEF1)及β-tubulin的核酸序列以最大似然法(maximum likelihood; ML)進行親緣關係樹的重建分析,結果顯示本菌株於3個基因所重建之親緣關係樹均歸類於Isaria javanica的支系。因此,根據型態及分子鑑定,本菌株應屬於I. javanica。以I. javanica CAES1之分生孢子處理咖啡果小蠹,7 d後之累計死亡率為100%,而觀察此菌感染後之產孢過程則發現,此菌於咖啡果小蠹死亡後約2.77 d ± 0.17 d即可於蟲體表面產生分生孢子。本研究為首篇於台灣之咖啡果小蠹上發現I. javanica之報告,且本菌對咖啡果小蠹具致病力,未來具有應用於田間防治咖啡果小蠹之潛力。 |
英文摘要 | An isolate of entomopathogenic fungi, CAES1, was isolated from a naturally infected coffee berry borer (Hypothenemus hampei; CBB) in a coffee field in Taiwan. Colonies of CAES1 were white to pale-yellow or grey. Mycelia could grow in range of 10℃ to 30℃, with the optimal temperature at 25℃, ranging from 10℃ to 30℃. The conidiophores formed branches with phialides in whorls of 1 to 4, with phialides 3.7-5.0-6.4 μm × 1.9-3.0-3.5 μm, and conidia forming in chain, 2.7-3.8-5.4 μm × 1.2-1.6-2.1 μm. CAES1 was identified as Isaria javanica based on morphology and phylogenetic analysis of the sequences of ribosomal internal transcribed spacer (ITS), translation elongation factor-1α (TEF1) and β-tubulin. The cumulative mortality rate of CBB was 100% on the 7th day after treated with conidia of I. javanica CAES1. I. javanica CAES1 sporulated on the surface of CBB 2.77 d ± 0.17 d after CBB died. This is the first record of I. javanica from CBB in Taiwan. It is suggested that I. javanica CAES1 is pathogenetic to the CBB and could be a potential biocontrol agent against CBB in the future. |
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