頁籤選單縮合
題 名 | Rab11-mediated Focal Adhesion Turnover in Sarcoma Cell Migration |
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作 者 | Kao, Ling-yi; Chao, Wei-ting; | 書刊名 | The Chinese Journal of Physiology |
卷 期 | 64:1 2021.01-02[民110.01-02] |
頁 次 | 頁43-50 |
分類號 | 415.138 |
關鍵詞 | Cell migration; Focal adhesion assembly; Focal adhesion kinase; Integrin; Rab11; Recycling endosome; |
語 文 | 英文(English) |
英文摘要 | Focal adhesion (FA) turnover has been demonstrated to play an important role in cell migration; however, the mechanism of FA turnover is complicated and requires further investigation. In this study, Rab11, which is involved in endosome recycling, was examined in terms of a direct regulatory function in FA formation during cell migration. Wild-type and dominant negative (DN) Rab11 or shRab11 were transfected into human HT1080 fibrosarcoma cells; the cell motility and migration abilities were determined, and localization of Rab11 and FA molecules was monitored by confocal microscopy. The results showed that Rab11 deficiency or the DN form inhibited sarcoma cell migration. Rab11 was also found to be co-localized with recycled β1 integrin and affected FA formation. We further employed immunofluorescence and immunoprecipitation to examine the physical interaction between Rab11 and focal adhesion kinase (FAK), and the results suggested that Rab11 affected cell migration by regulating FAK recycling to aid formation of an FA complex on the cell membrane. |
本系統中英文摘要資訊取自各篇刊載內容。