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題名 | Mesenchymal Stem Cells and Their Conditioned Medium Can Enhance the Repair of Uterine Defects in a Rat Model= |
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作者 | Ho, Chi-hong; Lan, Chen-wei; Liao, Chen-yi; Hung, Shih-chieh; Li, Hsin-yang; Sung, Yen-jen; Liao, Chen-yi; Sung, Yen-jen; Li, Hsin-yang; Ho, Chi-hong; Lan, Chen-wei; Hung, Shih-chieh; |
期刊 | Journal of the Chinese Medical Association |
出版日期 | 20180300 |
卷期 | 81:3 2018.03[民107.03] |
頁次 | 頁268-276 |
分類號 | 368.5 |
語文 | eng |
關鍵詞 | Mesenchymal stem cells; Paracrine effects; Transplantation; Uterine defect repair; Uterine surgery; |
英文摘要 | Background: Our aim was to examine the roles of mesenchymal stem cell (MSC) transplantation in the repair of large uterine defects. Methods: Uterine defects were created in both uterine horns of female rats by a punch instrument, and bone marrow-derived MSCs, MSCconditioned medium (MSC-CM) or vehicle were injected into the myometrium around the defect. The rate of uterine defect repair was monitored on day 2 and 4 after operation. Cytokine array of MSC-CM was performed, followed by neutralizing antibody experiments to clarify the exact cytokine participating in the MSC-CM-enhanced wound repair. Results: Transplantation of MSCs, but not myometrial cells, significantly enhanced uterine defect repair. The transplanted MSCs were detected in the uterine horn with no signs of rejection on day 4 after transplantation, when the MSC-transplanted uterine wound was nearly healed. Moreover, uterine defect repair was also accelerated by injection of MSC-CM, indicating the paracrine effects of MSCs on uterine wound healing. Cytokine array analysis further revealed that MSC-CM contained abundant cytokines and chemokines, among which high levels of interleukin-6 (IL-6) were found. Additionally, antibodies against IL-6 were shown to block MSC-CM-enhanced uterine defect repair. Conclusion: This study demonstrated that transplantation of MSCs could enhance uterine defect repair by paracrine effects involving IL-6, which are findings that may be applied to facilitate uterine wound healing in the removal of huge intramural masses. |
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