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頁籤選單縮合
| 題 名 | 建立基因毒理試驗對照組參考值範圍=Establishment of the Reference Ranges for Control Groups Used for Genotoxigenic Examinations |
|---|---|
| 作 者 | 周宜靜; 林彥君; 蔡岳廷; | 書刊名 | 檢驗及品保雜誌 |
| 卷 期 | 4:2 2015.05[民104.05] |
| 頁 次 | 頁78-84 |
| 分類號 | 415.132 |
| 關鍵詞 | 基因毒理; 自然回復突變; 染色體異常分析; 微核試驗; 對照組參考值; Ranges for cell genotoxicological analysis; Chromosome aberration; Mammalian erythrocyte micronucleus test; Reference results for control groups; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 利用本研究室過去的基因毒理試驗中微生物自然回復突變測試、細胞基因毒性分析與動物微核試驗的陽性及陰性對照組與文獻數據進行比較,以便了解實驗背景與實驗條件的關係及各國法規規範的異同。依據歷史數據建立對照組參考值的範圍,結果指出比較微生物自然回復突變測試的歷史數據與文獻數據後,發現本研究室所獲得之陰性對照組範圍略低,表示不易回復突變;而陽性對照組則較高,表示對陽性物質專一性高。細胞基因毒性分析結果,陰性對照組染色體異常率約在0~1.5%之間,陽性對照組則在2~17%之間。動物微核試驗陰性對照組微核發生率約在千分之0~1;陽性對照組微核發生率約千分之14~24,此範圍的建立使日後能快速經統計分析及判讀試驗結果是否在正常範圍內。綜合上述本研究室基因毒理試驗之歷史數據與文獻並無顯著差異,指出本研究室之檢驗結果具有可信賴性及參考價值,能作為相同條件下對照組之參考。 |
| 英文摘要 | In order to understand the differences between the regulations used in different countries and the relationships between experimental backgrounds and conditions, we compared the data obtained for positive and negative controls in past genotoxicological examinations, including Ames tests (microbial reversion tests), animal chromosome aberration tests and mammalian erythrocyte micronucleus tests, with the reference data published in the Journal. We established the range for these control groups using the historical data from our previous studies. Comparing our historical Ames test data with the published reference data, we found that the range for the negative control group was slightly lower, indicating that the phenomenon of reverse mutation did not occur easily. We also found that the range indicated for the positive control group in our historical data was higher than the range given in the published reference data, indicated a high specificity for positive control reference materials. The ranges for cell genotoxicological analysis showed that the chromosome aberration rate of the negative control group was approximately 0~1.5%, while that of the positive control group was 2~17%. Results also indicated that the micronucleus occurrence rate in the negative control group for the mammalian erythrocyte micronucleus test was about 0~1 per mille, while that of the positive control group was 14~24 per mille. The established range will subsequently help us to process the statistical analysis quickly and can be applied to determine whether a test result falls within the normal range. Based on the above findings, we can conclude that there were no significant differences between our historical laboratory data and the published reference data for the aforementioned genotoxicological tests, which would also indicate that the test results we obtained were reliable and could be valuable if used as reference results for control groups tested under the same conditions. |
本系統中英文摘要資訊取自各篇刊載內容。