頁籤選單縮合
題名 | Recombinant Outer Membrane Protein A Fragments Protect Against Escherichia coli Meningitis= |
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作者 | Hsieh, Wen-shyang; Yang, Yi-yuan; Yang, Hsin-yi; Huang, Yu-shan; Wu, Hsueh-hsia; |
期刊 | Journal of Microbiology, Immunology and Infection |
出版日期 | 20160600 |
卷期 | 49:3 2016.06[民105.06] |
頁次 | 頁329-334 |
分類號 | 415.9313 |
語文 | eng |
關鍵詞 | Bacterial meningitis; Escherichia coli; Outer membrane protein A; OmpA; |
英文摘要 | Background: Although the mortality rates have decreased over the past few decades, neonatal meningitis is still a severe disease with high morbidity. Moreover, approximately 40% of survivors exhibit neurological sequelae. Escherichia coli is the major Gram-negative bacterial pathogen in neonatal meningitis. The N-terminal b-barrel domain of the outer membrane protein A (OmpA) of E. coli is essential for effective protein conformation and function and contains four surface-exposed hydrophilic loops. In this study, we expressed different fragments of the four ring structures of the N-terminal domain, and investigated whether these recombinant OmpA fragments can protect mice from death after E. coli infection. Methods: We expressed the recombinant proteins of the following OmpA fragments by using molecular cloning of Loop 1e2, Loop 1e3, Loop 1e4, Loop 2e3, Loop 2e4, and Loop 3e4. Animal experiments were subsequently performed to investigate the effects of these recombinant OmpA fragments on the survival of C57BL/6 mice after intracerebral E. coli RS218 administration. Results: This study demonstrated that the recombinant Loop 1e3, Loop 2e3, and Loop 2e4 fragments of OmpA can protect mice from intracerebral E. coli infection. Conclusion: In bacterial meningitis, although antibiotic therapy is the first choice for management, neurological complications can seldom be averted. Based on the results of the present study, we intend to establish an effective therapeutic application for E. coli meningitis. |
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