頁籤選單縮合
題名 | 野鳥西尼羅熱之檢測方法建立及2006年臺灣監測結果=Development of Molecular Diagnostic Methods for Detecting West Nile Virus in Wild Birds in Taiwan in 2006 |
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作者 | 劉玉彬; 張光正; 鄭明珠; 李敏旭; 陳麗璇; 李淑慧; Liu, Y. P.; Chang, G. J.; Cheng, M. C.; Lee, M. S.; Chen, L. H.; Lee, S. H.; |
期刊 | 行政院農業委員會家畜衛生試驗所研究報告 |
出版日期 | 20071200 |
卷期 | 42 2007.12[民96.12] |
頁次 | 頁51-59 |
分類號 | 437.246 |
語文 | chi |
關鍵詞 | 西尼羅熱; 野鳥; 即時聚合酶鏈反應; West Nile fever; Wild birds; Real-time polymerase chain reaction; |
中文摘要 | 西尼羅熱(West Nile fever)為重要之人畜共通傳染病之一,為防範此新興疾病之入侵,建立早期預警監測系統為首要之務,其中又以對野鳥進行帶毒監測,為最敏感之偵測預警方法。家畜衛生試驗所疫學研究組已成功建立Nested PCR、SYBR Green I real-time PCR及TaqMan real-time PCR三種分子生物學診斷技術。Nested PCR最低可檢測5 copies之病毒核酸,較一般PCR敏感約1000倍;SYBR Green I real-time PCR亦具有相似之敏感性,可經由建立之核酸與循環數之檢量線,作為核酸定量之用;TaqMan real-time PCR則可以成功的區別西尼羅熱、日本腦炎(Japanese encephalitis)、聖路易氏腦炎(St. Louis encephalitis)及黃熱病(Yellow fever)四種黃病毒屬病毒。完成2006年野鳥樣本4,626件西尼羅病毒 之監測,檢測結果均為陰性。 |
英文摘要 | West Nile virus (WNV) infection is an important zoonosis. To prevent the possible introduction of this emerging disease into Taiwan, we have established a comprehensive wild-bird surveillance system for monitoring the presence of West Nile viral RNAs in migratory birds. Three detection methods for West Nile viral RNA, including nested PCR, SYBR Green I real-time PCR and Multiplex TaqMan real-time PCR, were used for this study. The nested PCR assay could detect 5 RNA copies, and it was 1000-fold more sensitive than that of traditional PCR. The sensitivity of SYBR Green I real-time PCR assay was equal to nested PCR. However, if the standard curve generated from known copy-number control specimens was included in the assay, the SYBR Green assay could be used to quantify the copy number of testing specimens. The multiplex TaqMan real-time PCR could differentiate four important flaviviruses, including WNV, JE, SLE and YF viruses. We have incorporated the surveillance project of WNV into the avian influenza surveillance project established previously. A total of 4,626 specimens from wild birds were processed in 2006, and none of them was WNV positive. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。