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題名 | Early Identification of Microorganisms in Blood Culture Prior to the Detection of a Positive Signal in the BACTEC FX System Using Matrix-assisted Laser Desorption/ionization--Time of Flight Mass Spectrometry= |
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作者 | Wang, Ming-cheng; Lin, Wei-hung; Yan, Jing-jou; Fang, Hsin-yi; Kuo, Te-hui; Tseng, Chin-chung; Wu, Jiunn-jong; |
期刊 | Journal of Microbiology, Immunology and Infection |
出版日期 | 20150800 |
卷期 | 48:4 2015.08[民104.08] |
頁次 | 頁419-424 |
分類號 | 414.7 |
語文 | eng |
關鍵詞 | Blood culture system; Blood stream infection; Mass spectrometry; Matrix-assisted laser desorption ionization–time of flight mass spectrometry; Time to detection; |
英文摘要 | Background: Matrix-assisted laser desorption/ionizationetime of flight mass spectrometry (MALDI-TOF MS) is a valuable method for rapid identification of blood stream infection (BSI) pathogens. Integration of MALDI-TOF MS and blood culture system can speed the identification of causative BSI microorganisms. Materials and methods: We investigated the minimal microorganism concentrations of common BSI pathogens required for positive blood culture using BACTEC FX and for positive identification using MALDI-TOF MS. The time to detection with positive BACTEC FX and minimal incubation time with positive MALDI-TOF MS identification were determined for earlier identification of common BSI pathogens. Results: The minimal microorganism concentrations required for positive blood culture using BACTEC FX were >107e108 colony forming units/mL for most of the BSI pathogens. The minimal microorganism concentrations required for identification using MALDI-TOF MS were > 107 colony forming units/mL. Using simulated BSI models, one can obtain enough bacterial concentration from blood culture bottles for successful identification of five common Gram-positive and Gram-negative bacteria using MALDI-TOF MS 1.7e2.3 hours earlier than the usual time to detection in blood culture systems. Conclusion: This study provides an approach to earlier identification of BSI pathogens prior to the detection of a positive signal in the blood culture system using MALDI-TOF MS, compared to current methods. It can speed the time for identification of BSI pathogens and may have benefits of earlier therapy choice and on patient outcome. |
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