頁籤選單縮合
題名 | 臺灣本土中草藥之應用與開發--臺灣鉤藤=Development and Utilization of Indigenous Taiwanese Herbal Medicine--Uncaria Hirsuta Haviland |
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作者 | 李美賢; 侯文琪; 林乾閔; Lee, Mei-hsien; Hou, Wen-chi; Lin, Chien-min; |
期刊 | 中醫藥年報 |
出版日期 | 20141200 |
卷期 | 3 2014.12[民103.12] |
頁次 | 頁(61)0-(61)29 |
分類號 | 418.5 |
語文 | chi |
關鍵詞 | 臺灣本土中草藥; 臺灣鉤藤; 神經保護; Taiwanese herbal medicine; Uncaria hirsuta; Neuroprotection; |
中文摘要 | 中藥材鉤藤為為茜草科 (Rubiaceae)植物,基源主要是中國大陸的 Uncaria rhynchophylla (Miq.) Jacks.,屬於平肝熄風藥。藥效為清熱平肝,熄風定驚,主要用在清熱祛風,外感風熱,小兒驚癇,血壓偏高,頭昏目眩。本計畫以台灣本土中草藥之應用與開發為主要目標,為達成此目標,我們選擇農委會種苗改良繁殖場提供之田間種植的台灣本土中草藥 —臺灣鉤藤 (Uncaria hirsuta Haviland)為材料,進行其活性成分分析及神經保護之探討及應用。並比較鉤藤與臺灣鉤藤於活性成分及神經保護作用之異同,達到應用台灣本土中草藥之目標。臺灣鉤藤之材料來源經由 PCR-RFLP檢測確定無誤,並進行總重金屬含量與農藥殘留分析,確保品質合格後,進行研究分析。利用水與 95%乙醇以加熱迴流萃取法,分別進行中藥材鉤藤帶鉤莖枝、臺灣鉤藤帶鉤莖枝及葉部粗萃取物製備。利用薄層色層分析、高效能液相層析與高效能液相層析儀-固相萃取儀-核磁共振儀進行成分之分析與比較,臺灣鉤藤葉部之水粗萃取物之化學成分與中藥材鉤藤有明顯不同,含有其他多種 indole alkaloids之成分。在被動迴避試驗的結果來看,半乳糖誘導確實可以引發小鼠記憶衰退的現象,而投藥的各粗萃物組停留在明亮室的時間明顯高於半乳糖誘發之實驗對照組,顯示臺灣鉤藤及藥用鉤藤的藥部粗萃物在記憶能力的鞏固上有其效果。但在水迷宮試驗則沒有明顯效果。而在脂質過氧化指標的表現上,不論在血液中或是腦中,經由半乳糖誘導後的小鼠,丙二醛含量皆較正常小鼠多,顯示半乳糖會誘發脂質過氧化增加,因而表現於被動迴避試驗中小鼠記憶衰退的現象;而在投與臺灣鉤藤及藥用鉤藤粗萃物後,丙二醛含量有下降的現象,顯示其減少脂質過氧化的能力;其具體表現在各粗萃物組停留在明亮室的時間明顯高於半乳糖誘發之實驗對照組。在細胞實驗上,臺灣鉤藤葉部水萃物及藥用鉤藤藥部水萃物本身而言有較小的細胞毒性,同時對抗 6-羥基多巴胺誘發之細胞毒性上有明顯增加細胞存活率的現象,顯示其神經保護的能力。但在 A 25-35引發的細胞毒性實驗上,則沒有顯著的效果。 |
英文摘要 | Chinese herbal medicine – Uncaria Ramulus et Uncus is a Rubiaceae plant. The sources in mainland China is mainly Uncaria rhynchophylla (Miq.) Jacks. In traditional utility, it belongs to anticonvulsives or drugs for subduling the hyperactivity of the liver and the endogenous wind. Efficacy for clearing up heat, subduing hyperactivity of the liver, and relieving convulsion. It mainly used in heat dispelling, exogenous wind-heat, shock in children epilepsy, high blood pressure, dizziness. In this project, the application of Taiwanese Chinese herbal medicine development as the main objective, to achieve this goal, we chose Uncaria hirsuta Haviland from Taiwan Seed Improvement and Propagation Station, COA as the material. The active ingredient extraction, neural protection, and its application will be evaluated in this project. And comparing with the active ingredients and neuroprotective effects of the two materials from different sources was also included. Upon completion, we hope to achieve the goal of application of Taiwanese herbs. The material source of Uncaria hirsuta Haviland was identified by PCR-RFLP. The total heavy metals and pesticide residues of U. hirsuta were also analyzed and to ensure the quality. The hooks of U. rhynchophylla, the hooks and leaves of U. hirsuta were refluxed with H2O and 95% ethanol, respectively. The use of in vitro model as a platform for activity assessment of the extracts. The leaves of water extract of U. hirsuta exhibited the potent activity. The analysis of thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) for U. hirsuta were used as chemical chromatographic fingerprints. We further investigated the constituents from active fractions of U. hirsuta by high performance liquid chromatography–solid phase extraction–nuclear magnetic resonance (LC-SPE-NMR). The fraction contained some indole alkaloids which were different from the U. rhynchophylla. In passive avoidance task of galactose-induced mice, it was found that galactose could induce aging and resulted in memory impairments. The oral administrations of extracts from U. hirsuta or U. rhynchophylla to galactose-induced mice could significantly improve memory impairments in passive avoidance task, but not observed in Morris water maze model. The lipid peroxidation index of MDA contents were significantly increased in blood or brain tissue extracts of galactose-induced mice, and the oral administrations of extracts from U. hirsuta or U. rhynchophylla could significantly lower the MDA contents. It meant that extracts from U. hirsuta or U. rhynchophylla colud reduce lipid peroxidation levels in galactose-induced mice which in turn to improve memory impairments in mice. For elucidation of possible protective mechanisms, the SH-SY5Y cells were used in protection against 6-OH dopamine or Aβ 25-35 treatments. It was found that water extracts from leaves of U. hirsute or U. rhynchophylla showed less cytotoxicity and also had protective roles against 50 µM 6-OH dopamine, but had no effects against Aβ25-35 induced cytotoxicity. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。