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題名 | Response Surface Optimization of Microbial Prodigiosin Production from Serratia Marcescens |
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作者 | Su, Wen-ta; Tsou, Tsung-yu; Liu, Hsuan-liang; | 書刊名 | Journal of the Taiwan Institute of Chemical Engineers |
卷期 | 42:2 2011.03[民100.03] |
頁次 | 頁217-222 |
分類號 | 368.87 |
關鍵詞 | Prodigiosin; Serratia marcescens; Experimental design; Optimization; Response surface methodology; |
語文 | 英文(English) |
英文摘要 | Abstract A gram-negative bacterium isolated from soap (identified as Serratia marcescens) can produce microbial red-pigmented prodigiosin. The prodigiosin was extracted using acetone after 24 h cultivation period. The results revealed that the addition of 0.5% sucrose and 0.5% glycine in cultivated medium can enhance prodigiosin production, increasing 2.12-fold and 2.15-fold, respectively. Moreover, an addition of inorganic salt KH2PO4 may increase 1.17-fold prodigiosin production as compared with control. The statistical experimental design was used to optimize the medium constituents for maximal prodigiosin production by S. marcescens. The experimental results showed that the optimal medium constituents were 0.5% glycine, 0.454% peptone, 1626.3 ppm KH2PO4, and 0.5% sucrose. Under this optimal composition, the predicted prodigiosin production was 2230.2 mg/L, and the experimental value was 2423.4 mg/L for 1 day culture in shake flasks. The microbial prodigiosin productivity was enhanced by nearly 8.42-fold (from 273.3 to 2423.39 mg/L). |
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