頁籤選單縮合
題 名 | 枯草桿菌Bacillus subtilis BS-99-H之Iturin A抗菌物質對蓮霧果腐病原菌Pestalotiopsis eugeniae之生物活性=Bioactivity of Antifungal Substance Iturin A Produced by Bacillus subtilis Strain BS-99-H against Pestalotiopsis eugeniae, a Causal Pathogen of Wax Apple Fruit Rot |
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作 者 | 林秀芬; 陳滄海; 劉顯達; | 書刊名 | 植物病理學會刊 |
卷 期 | 19:3 2010.09[民99.09] |
頁 次 | 頁225-233 |
分類號 | 433.85 |
關鍵詞 | 枯草桿菌BS-99-H; 蓮霧果腐病原菌; Iturin A; Bacillus subtilis BS-99-H; Pestalotiopsis eugeniae; |
語 文 | 中文(Chinese) |
中文摘要 | 從玻璃紙抗生法的研究顯示,令枯草桿菌(Bacillussubtilis)BS-99-H之瓊脂塊放在玻璃紙上,再置於培養基平板上培養1-4天,去除含有枯草桿菌玻璃紙之培養基經90℃加熱10分鐘及未經加熱處理,皆對蓮霧果腐病原菌(Pestalotiopsiseugeniae)的菌絲生長有相同的抑制效 果,至培養第5天之後,對P.eugeniae菌絲生長的抑制率達100%。由上述具有拮抗作用之培養基中萃取可能的抗菌物質,經HPLC及串聯式質譜儀(MS/MS)分析得知其為iturinA抗生物質。另將部分純化iturinA與P.eugeniae菌絲混合處理後,發現菌體細胞導電度於8小時後顯著增加3.4倍,至28小時達到高峰,為5.3倍;以顯微鏡觀察P.eugeniae菌絲呈現異常膨脹與扭曲。BS-99-H所產生的iturinA抑制P.eugeniae孢子發芽之IC50為0.51mg/ml,當iturinA濃度為≥0.84mg/ml則完全抑制P.eugeniae孢子發芽與菌絲生長。從本研究結果顯示出BS-99-H 產生的iturinA是抑制P.eugeniae很重要的因子,在發展對P.eugeniae之生物防治上將可扮演 重要的角色,因此本研究所得的枯草桿菌BS-99-H未來具有潛力開發作為蓮霧果腐病害之生物性殺菌劑。 |
英文摘要 | Using the cellophane paper antibiosis method, an agar disc containing Bacillus subtilis BS-99-H was placed on a cellophane paper which was overlaid on the surface of an agar medium plate. After incubation, the cellophane paper was removed, and the medium that was heated at 90℃ for 10 min or non-heated was assayed for the inhibitory activity against mycelial growth of Pestalotiopsis eugeniae. Inhibition of mycelial growth of P. eugeniae was observed on both heated and non-heated media one day after incubation, and a complete inhibition can be observed at 5 days post incubation. The extracts from the heated and non-heated media were analyzed by the HPLC and tandem mass spectrometry (MS/MS) methods, and iturin A was detected from both media. The partially purified iturin A was found to cause electrolyte leakage of P. eugeniae mycelia. The leakage was increased 3.4 folds at 8 hours post treatment and reached maximum increase of 5.3 folds at 28 hours post treatment. Treatment with the BS-99-H iturin A also caused swelling and malformed shape of the hyphae of P. eugeniae. The IC50 value of iturin A from BS-99-H for inhibition of P. eugeniae conidial germination was 0.51 mg/ml. The conidial germination and mycelia growth were both completely inhibited by iturin A at ≥ 0.84 mg/ml. The above results indicate that antibiotic iturin A produced by BS-99-H played an important role in antagonism against P. eugeniae. Therefore, B. subtilis BS-99-H could be a useful organism for development of a biofungicide agent for biocontrol of wax apple fruit rot. |
本系統中英文摘要資訊取自各篇刊載內容。