頁籤選單縮合
| 題 名 | Behind the Scenes of an Ant Genome Project=火蟻基因組計畫的幕後側寫 |
|---|---|
| 作 者 | Wurm, Yannick; | 書刊名 | 臺灣昆蟲 |
| 卷 期 | 31:2 2011.06[民100.06] |
| 頁 次 | 頁149-156 |
| 分類號 | 387.781 |
| 關鍵詞 | 基因體組裝; 指引; 基因體註解; 火蟻; 入侵紅火蟻; Genome assembly; How-to; Genome annotation; Fire ant; Solenopsis invicta; |
| 語 文 | 英文(English) |
| 中文摘要 | 近五年DNA定序技術的進展已使得定序成本比過去減少1,000倍以上。因此全基因體研究的方法不僅應用於解決醫學相關問題,也能進一步探討大部分生物的基礎生物學,包括以分子遺傳為主之行為模式、發育及獨特之活史。以新興模式生物為例,首要步驟即為建立參考基因組序列。本文將提供以火蟻基因體定序計畫所得之啟發。首先,詳述此計畫的緣由,並介紹如何選擇基因體解序、組裝和基因體註解的策略。接著說明如何將火蟻基因體資料與近來被解序之物種作結合。最後,討論以火蟻基因體序列為基礎,進行後續研究計畫的方法。 |
| 英文摘要 | Dramatic improvements in DNA sequencing technologies have led to a more than 1,000-fold reduction in sequencing costs over the past five years. Genome-wide research approaches can thus now be applied beyond medically relevant questions to examine the molecular-genetic basis of behavior, development and unique life histories in almost any organism. A first step for an emerging model organism is usually establishing a reference genome sequence. I offer insight gained from the fire ant genome project. First, I detail how the project came to be and how sequencing, assembly and annotation strategies were chosen. Subsequently, I describe some of the issues linked to working with data from recently sequenced genomes. Finally, I discuss an approach undertaken in a follow-up project based on the fire ant genome sequence. |
本系統中英文摘要資訊取自各篇刊載內容。