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題名 | 黏土礦物對酵母菌與大腸桿菌表面電化學特性的影響=Electroanalysis of Surface Effect of Clay Minerals on Saccharomyces cerevisiae and Escherichia coli |
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作者 | 許得美; 陳鴻基; 曾志明; Hsu, De-mei; Chen, Horng-ji; Zen, Jyh-myng; |
期刊 | 台灣農學會報 |
出版日期 | 20160300 |
卷期 | 17:1 2016.03[民105.03] |
頁次 | 頁1-22 |
分類號 | 434.223 |
語文 | chi |
關鍵詞 | 黏粒修飾電極; 酵母菌; 大腸桿菌; 電化學分析; Clay-modified electrode; Saccharomyces cerevisiae; Escherichia coli; Electroanalysis; |
中文摘要 | 黏土礦物與微生物的交互作用對土壤中的化學反應與生態平衡扮演非常重要的角色,以簡單、迅速之黏粒修飾電極搭配循環伏安法來探討微生物與黏土礦物的交互作用及表面電化學特性。試驗結果指出,由Ru(NH_3)_6^(3+)在兩種菌膜層的氧化還原波峰都隨著掃描圈數的增加而有逐漸增大情形,可看出兩種菌表面帶有負電荷的特性。六種黏土礦物與酵母菌的交互作用使Fe(CN)_6^(3-)的還原波峰由第一圈到第五圈有逐漸減少的現象,而氧化波峰則無此情形,六種黏土礦物中以PFl-1的影響最為明顯,且由電子顯微鏡圖中可看出PFl-1的絲狀結晶構造包裹在酵母菌的表面。六種黏土礦物與大腸桿菌的交互作用對Fe(CN)_6^(3-)電化學行為的影響也有相同的情況,且其中以KGa-1和PFl-1的影響較大。因此,以Fe(CN)_6^(3-)為探針化合物的電化學方法可分辨出兩種菌表面的電荷性及電化學特性差異。 |
英文摘要 | The interaction between clay minerals and microbes has an important role in the chemical reactions and ecological balance of soils. We report here the interaction of clay minerals and microbes by a simple electrochemical method based on a clay-modified electrode. The results of cyclic voltammetry indicated an increasing redox peaks of Ru(NH_3)_6^(3+) at the microbe-coated clay-modified electrode. This indicates a negative charge character of these microbes' surface. More specifically, the PFl-1 clay apparently affects Saccharomyces cerevisiae to cause a regular decrease of the reduction peak of Fe(CN)_6^(3-) from the first to fifth cycle. Similarly, Escherichia coli also have the same effect caused by KGa-1 and PFl-1. Field emission SEM further illustrates that the fibrous mineral crystals of PFl-1 were coated on the S. cerevisiae surface. Most importantly, the different electrochemical character of two microbes could be distinguished by the electroanalysis of Fe(CN)_6^(3-) at microbe-coated clay-modified electrode. |
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