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題 名 | Fermentation, Biodegradation and Tensile Strength of Poly (3-hydroxybutyrate-co-4-hydroxybutyrate) Synthesized by Delftia Acidovorans |
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作 者 | Hsieh, Wen-chuan; Wada, Yuki; Chang, Chih-pong; | 書刊名 | Journal of the Taiwan Institute of Chemical Engineers |
卷 期 | 40:2 2009.03[民98.03] |
頁 次 | 頁143-147 |
分類號 | 460.02 |
關鍵詞 | Fermentation; Biodegradation; Tensile strength; P (3HB-co-4HB); Poly (3-hydroxybutyrate-co-4-hydroxybutyrate); |
語 文 | 英文(English) |
英文摘要 | Abstract This study discusses poly(3-hydroxybutyrate-co-4-hydroxybutyrate) that is produced by Delftia acidovorans bacteria in fed-batch cultures from various carbon sources. The experimentally determined optimal fed-batch culture conditions are as follows: pH: 7.0, incubation temperature: 26 °C, incubation time: 72 h, and concentration of carbon source: 10 g/L. The maximum 4HB unit composition of P(3HB-co-4HB) obtained using the fed-batch culture with a cell concentration of about 2.5 g/L was 94 mol% although the P(3HB-co-4HB) content remained low, at around 13 wt%. The extent of the enzymatic degradation of P(3HB-co-4HB) was determined by involved PHB depolymerase and lipase. The tensile strength of P(3HB-co-4HB) markedly decreased during 2 h of degradation by PHB depolymerase, whereas that of P(3HB-co-4HB) degraded by lipase for 50 h was at least 3 MPa. |
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