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題名 | 皂角刺萃取物對於溶血性與致突變性試驗之研究=Study of Gleditsia Sinensis Thorns Extract on the Hemolysis and Mutagenic Test |
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作者 | 曾文楷; 陳宜嫻 ; 官常慶; | 書刊名 | 美容科技學刊 |
卷期 | 5:2 2008.09[民97.09] |
頁次 | 頁57-70 |
分類號 | 414.33 |
關鍵詞 | 皂角刺; 溶血; 致突變性; 抗致突變性; Gleditsia sinensis thorns; Hemolysis; Mutagenicity; Antimutagenicity; |
語文 | 中文(Chinese) |
中文摘要 | 本研究的目的是探討皂角刺的安全性,以評估其作為化粧品原料的可行性。將皂角刺經95%乙酵萃取,所得的皂角刺乙醇萃取物採用非實驗動物試驗法進行測試。如溶血性試驗和沙門氏菌回復突變性試驗,分別作為評估眼睛刺激性與基因毒性(或致突變性或致癌性)的安全性試驗法。此外,並探討皂角刺乙醇萃取物對4-nitroquinoline-N-oxide(4-NQO)致突變之抗致突變性。本研究中皂角刺萃取物的製備,所得皂角刺乙醇萃取物的萃取率為2.47%。由溶血性試驗的結果顯示:皂角刺乙醇萃取物之HC50為547.404μg/ml,且皂角刺乙醇萃取物的log HC50=2.738,大於Sodium lauryl sulfate的logHC50=1.895,表示皂角刺乙醇萃取物不具眼睛刺激性的可能性很高。由沙門氏菌回復突變性試驗的結果顯示:皂角刺乙醇萃取物對沙門氏菌TA98與沙門氏菌TA100無毒性之劑量分別為小於或等於0.5mg/plate和1mg/plate,以皂角刺乙醇萃取物對菌株無毒性的濃度以下,再進行對菌株的致突變性測試,結果顯示皂角刺乙醇萃取物對沙門氏菌TA98與沙門氏菌TA100所測試之回復菌落數皆小於控制組的二倍,即表示皂角刺乙醇萃取物不具有致突變性。由抗致突變性試驗的結果顯示:皂角刺乙醇萃取物0.1mg/plate對4-NQO引起致沙門氏菌TA98突變的抑制率為72.73%,對4-NQO引起致沙門氏菌TA100突變的抑制率為61.92%,均屬於高度活性的抑制作用(抑制率≥60%),表示皂角刺乙醇萃取物具有良好的抗致突變作用。 |
英文摘要 | The purposes of this research are probing into the safety and evaluating the feasibility as a cosmetic raw material of Gleditsia sinensis thorns. The Gleditsia sinensis thorns were extracted by ethanol (95%), and the ethanol extract of Gleditsia sinensis thorns were used in alternative methods to animal tests. These tests included hemolysis test and Ames test which are used for evaluating the safety of the eye irritation and the genotoxicity (mutagenicity and/or carcinogenicity), respectively. Besides, the antimutagenicity of the ethanol extract of Gleditsia sinensis thorns against the mutagenicity of 4-nitroquinoline-N-oxide (4-NQO) in stains Salmonella typhimurium TA98 and TA100 were also examined The rate were 2.47% in the extraction of Gleditsia sinensis thorns by ethanol in this study. The results of hemolysis test indicate that HC50 of the ethanol extract of Gleditsia siensis thorns is 547.404μg/ml, i.e. log HC50=2.738 which is greater than that of sodium lauryl sulfate (log HC50=1.895). Therefore, it can be concluded that eye irritation caused by the ethanol extract of Gleditsia sinensis thorns is very low. The results of Ames test show that the concentrations of non-toxicity of the ethanol extract of Gleditsia sinensis thorns to Salmonella typhimurium TA98 and TA100 are 0.5 mg/plate and 1 mg/plate, respectively. Using the ethanol extract of Gleditsia sinensis thorns under the concentration of non-toxicity for the mutagenic test of strains, the ethanol extract of Gleditsia sinensis thorns smaller numbers of revertants of Salmonella typhimurium TA98 and TA100 than that of controlled groups by two times. These results suggest that the ethanol extract of Gleditsia sinensis thorns does not possess mutagenicity. The results of antimutagenic test indicate that the ethanol extract of Gleditsia sinensis thorns with 0.1 mg/plate gives suppressing rates of 72.73% and 61.92% for the mutation of Salmonella typhimurium TA98 and the mutation of Salmonella typhimurium TA100, respectively, caused by 4-NOQ. These results suggest that the ethanol extract of Gleditsia sinensis thorns shows a strong activity of antimutagenicity (suppressing rate is 60%) and is a good antimutagenic agent. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。