頁籤選單縮合
題名 | 不同蜜蜂種的蘋果酸脫氫酶II(MDH II)分析=Genetic Diversity of MDH II in Different Honeybee Species |
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作者 | 張學鋒; 吳小波; 羅嶽雄; 趙紅霞; 林琦; 張含; 曾志將; Zhang, Xue-feng; Wu, Xiao-bo; Luo, Yue-xiong; Zhao, Hong-xia; Lin, Qi; Zhang, Han; Zeng, Zhi-jiang; |
期刊 | 臺灣昆蟲 |
出版日期 | 20091000 |
卷期 | 29:3 2009.10[民98.10] |
頁次 | 頁165-171 |
分類號 | 438.71 |
語文 | chi |
關鍵詞 | 蜜蜂; 蘋果酸脫氫酶II; 遺傳多樣性; Honeybee; Malate dehydrogenase; Genetic diversity; |
中文摘要 | 為研究不同蜜蜂種的蘋果酸脫氫酶 II (MDH II) 的遺傳差異,採用聚丙烯醯胺 等電點聚焦技術 (IEF-PAGE),對大陸飼養的漿蜂 (“high royal jelly” Apis mellifera L.),江山意蜂 (“Jiangshan ” Apis mellifera L.),卡尼鄂拉蜂 (Apis mellifera carnica),東北黑蜂 (Apis mellifera ssp.),江西靖安意蜂 (”Jingan” Apis mellifera L.) 和 5 個不同蜂種的蘋果酸脫氫酶 II 的基因型、基因型頻率、基因頻 率、雜合度和純合度的進行了分析。結果表明:不同西方蜜蜂 MDH II 存在豐富的 遺傳多樣性,卡尼鄂拉蜂基因頻率 a 最高,達 79.7%。江山本意基因頻率 b 最高, 為 53.8%,漿蜂基因頻率 c 最高為 64.2%。漿蜂雜合度最高為 67.3%,最低為卡尼 鄂拉蜂 30.5%;不同西方蜂種之間的基因型頻率、基因頻率、雜合度和純合度存在極 顯著差異。 |
英文摘要 | This paper investigated the genetic diversity of MDH II in 5 different honeybee species by means of IEF-PAGE technology. The genotype, genotype frequency, allele frequency, as well as the homozygous and heterozygous degree of MDHI II were analyzed in this study, leading us to discover the polymorphism of MDH II in these western honeybees. Allelea was found to be the dominant loci in Apis mellifera carnica (79.7%), and the allele b frequency was predominant in Apis mellifera ligustica from Jiangshan (53.8%), while allele c showed to have a higher frequency in Apis mellifera ligustica of high royal jelly bee (64.2%) than the other. Genetic heterosis of the high royal jelly bee is the highest at 67.3%, but it was low in Apis mellifera carnica. These results showed to be significantly different among the different honeybees regarding genotype, genotype frequency, gene frequency, gene heterosis, and gene homogeneity of MDH II. |
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