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題名 | 臺灣西部溪流構樹之遺傳結構與水系棲地關係=Relationship between Population Genetic Structure and Riparian Habitats of Broussonetia Papyrifera Vent. on the Western Taiwan |
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作者 | 何坤益; 張景富; Ho, Kuenu-yih; Chang, Jin-fu; |
期刊 | 國立臺灣大學生物資源暨農學院實驗林研究報告 |
出版日期 | 20060900 |
卷期 | 20:3=253 民95.09 |
頁次 | 頁165-174 |
分類號 | 373.961 |
語文 | chi |
關鍵詞 | 構樹; 簡單重複序列; 遺傳分化; 基因流; 遺傳變異; Broussonetia papyrifera Vent; ISSR; Genetic diversity; Gene flow; Genetic variation; |
中文摘要 | 構樹(Broussonetia papyrifera Vent.)為桑科(Moraceae),分布在海拔約0-2000 m溪邊優勢植物,族群集中分佈在溪流環境內,基因流在不同水系間的交流機會較少,不同溪流間的族群遺傳結構可能會有較大的差異。本研究應用簡單重複序列(Inter-Simple Sequence Repeat, ISSR)分子指紋技術,探討臺灣西部五條水系的構樹族群遺傳結構,共採集18個族群180個樣本,利用13個引子(Primers)進行PCR試驗。POPGENE分析結果發現Nei's族群基因歧異度低,水系間族群分化差異明顯(H: 0.1624, Gst: 0.2476),水系族群之基因流順暢(Nm: 1.5192);AMOVA分析結果顯示,水系間變方成分百分比為24.21% (p<0.0001),水系內族群間變方成分百分比為13.01% (p<0.0001),水系間族群間變方成分百分比為62.78% (p<0.0001),顯示構樹族群在不同水系間分化之趨勢,但大部分的分化仍存在於族群內。就族群遺傳變異與地理距離間具顯著相關,但海拔無顯著差異。歸群及主座標分析結果可分成兩大群,高屏溪與其它水系族群的關係較遠,推測族群遺傳變異為棲地差異之影響。 |
英文摘要 | Broussonetia papyrifera Vent. of Moracea, is a native riparian and dominant tree species at the altitude from 0 to 2000m in Taiwan. Since gene flow is suspected to be restricted mainly within a single river system, we hypothesize that plants of each river system might have rather distinct genetic structure due to limited gene flow. In the present study, 180 individual samples from 18 sampling sites (populations) in five river systems were studied using ISSR markers. The variation of 93 polymorphic ISSR bands, 78 bands were polymorphic in the samples were recorded. Analysis of the population genetic variances demonstrated that there were unremarkable Nei's genetic diversity, and conspicuous population differentiation between different river systems (H=0.1624, Gst=0.2476), with high level of gene flow (Nm=1.5192). The results were probably due to that the influence from the different environments did not restrict the gene flow but promote a lower genetic differentiation. The analysis of molecular variance (AMOVA) revealed that the components of variance among river systems was 24.21% (p<0.001). Among populations within river systems was 13.01% (p<0.001). Main variance within populations, shown to be significant, was 62.78% (p<0.001). The results indicated that there is a considerable genetic diversification among river systems, and most of the diversification was between populations. Based on our mantel test, correlation coefficients among genetic variation among for 18 natural populations were significant, influenced by the geographical distribution but uninfluenced by the elevation. The cluster analysis and principal coordinate analysis revealed two major general groupings of northern and central-southern groups. The Gao-ping-shi river system was found to be a relatively isolated group by the cluster analysis. Cluster analysis and principal coordinate analysis (PCOA) also revealed that the genetic differentiation may be caused by different environments. |
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