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頁籤選單縮合
題名 | 初代無特定病原兔設施及種原建立之研究=Production of Primary Specific Pathogen Free Rabbits for Experimental Use |
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作者 | 林榮培; 陳玫雅; 邱顯閔; 梁奇鳳; 許天來; Lin, Y. P.; Chen, M. E.; Chiu, H. M.; Liang, C. F.; Hsu, Tien-lai; |
期刊 | 行政院農業委員會家畜衛生試驗所研究報告 |
出版日期 | 20071200 |
卷期 | 42 2007.12[民96.12] |
頁次 | 頁89-96 |
分類號 | 437.68 |
語文 | chi |
關鍵詞 | 無特定病原; 兔; 實驗動物; SPF; Rabbit; Laboratory animal; |
中文摘要 | 為提昇實驗用兔之品質至無特定病原(specific pathogen free;SPF)等級,本分所於93年2月新建完工乙棟正壓密閉之SPF實驗動物舍,進入之空氣先經高效率濾網濾除微塵與微生物,再經空調降溫至攝氏25℃。其中供SPF實驗用兔繁殖生產之用的面積約200坪,隔間設有手術室、繁殖室、種兔室、育成室、洗滌室、飼料間、穿牆式雙門滅菌鍋等。手術室備有無菌操作手術箱及動物隔離箱(animal isolator)等設備,以供初代SPF 實驗用兔生產之用。為生產初代SPF實驗用兔,選取傳統清淨等級懷孕母兔,以帝王切開術(Caesarean section)取出仔兔,置隔離箱實施人工哺乳,並於21日齡至33日齡投予Bacteroides等特定細菌或乳酸菌,以建立腸道正常菌群。其後,將仔兔從動物隔離箱移出飼養,所用的飼料、飲水及物品器具等均經滅菌處理,空氣經低、中、高效能之濾網過濾,採全換氣方式並保持室內正壓。總計進行五胎次剖腹產,成功育成初代SPF實驗用兔31隻。經健康監控與監測10種兔病結果皆陰性,顯示其健康合乎SPF兔之標準。這些初代SPF實驗用兔將進一步進行選育與二代SPF實驗用兔生產與供應。 |
英文摘要 | For upgrading the quality of experimental rabbits from conventional clean to specific pathogen free (SPF) levels, a new animal building for the production of SPF rabbits was established at the Branch Institute for Drugs Inspection, Animal Health Research Institute in 2004. This animal building has about 660 square meters in size and contains a closed system with positive air pressure system. The air entering the house is filtered by a high efficiency special air filter to prevent it from carrying pathogens and dusts, and the room temperature is kept at 25℃ by air-conditioning. Rooms inside the animal facility are allocated for surgery, breeding, breeder, nursery, wash and preparation, feed and through type steam sterilization. In the surgery room, biosafety cabinets for surgery and animal isolator are equipped for primary SPF production. For technical establishment and production of primary SPF rabbits, conventional clean does of New Zealand breed, at the last day of gestation or at the day of laboring, are selected for Caesarean delivery of kittens by aseptic techniques. The kittens are kept in animal isolator first and raised by artificial suckling method. On Day 21 to 33 after birth, kittens are fed with lactobacilli and bacteroides for developing normal intestinal flora. Then the rabbits are transferred from animal isolator to cage unit for further raising. Feed, water, and materials used for primary SPF rabbits are all sterilized. We have performed Caesarean section on five breeding does and got 31 SPF rabbits. The results of health surveillance and monitoring of primary SPF rabbits showed that the animals were free from 10 common rabbit pathogens, and this indicated that the health status of primary rabbits has met the SPF criteria. These primary SPF rabbits will be further bred to generate the secondary SPF rabbits. In addition to SPF rabbits of the Japanese breed, we now have a new choice of laboratory New Zealand breed SPF rabbits for research in biology, biotechnology and biomedicine in Taiwan. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。