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題名 | 以整合性生物製程生產酒精、丁醇、生質柴油等生質燃料並產出麩胱甘肽與大豆胚芽粉等生技原料=Production of Various Biofuels and Raw Biomaterials Using Integrated Bioprocesses |
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作者 | 林忠亮; 陳文章; 李旻倉; 林士民; 湯雅理; 張嘉修; Lin, Chung-liang; Chen, Wen-chang; Li, Min-cang; Lin, Shyh-mirn; Tang, Ya-li; Chang, Jo-shu; |
期刊 | 石油季刊 |
出版日期 | 20130600 |
卷期 | 49:2 2013.06[民102.06] |
頁次 | 頁47-64 |
分類號 | 400.15 |
語文 | chi |
關鍵詞 | 生質燃料; 生技原料; 整合性生物製程; 發酵; Biofuels; Raw biomaterials; Integrated bioprocesses; Fermentation; |
中文摘要 | 本研究係開發整合性生物製程分別以木薯澱粉及大豆為進料,利用酵母融合菌株以木薯澱粉為料源,依序以饋料-批次式發酵生產生質酒精及麩胱甘肽(Glutathione, GSH),於發酵生產生質酒精46小時後與繼續發酵生產GSH 36小時後,分別可得12.0%(wt%)生質酒精及19.4 mg/g-DCW GSH;再利用上述木薯澱粉發酵酒精之殘渣廢液為進料基質,以Cl. acetobutylicum S1厭氧發酵生產生質丁醇,其最大丁醇產量達3.1 g/L,丁醇產率為0.40 mol butanol/mol Sugar。另以大豆為料源利用機械設備以離心撞擊法進行大豆胚軸與子葉分離,其產率為1.66%,內含豐富的大豆異黃酮及多種生理活性物質,可做為生技保健產品主原料;將經分離大豆胚軸之子葉,使用己烷提取大豆油,其榨油率為15.1%。以此大豆粗油和上述所得之丁醇為料源,利用固定化R. oryzae BD5菌體進行轉酯化生產生質柴油,其製程之最適條件為:正丁醇-大豆油莫耳比=3、固定化菌體含量4 wt.%、含水量5 wt.%,以及反應時間為72 h,轉化率可達78.84 %。此固定化R. oryzae BD5菌體在連續使用4次之後,仍保有80%的活性。本整合性生物製程可生產生質酒精、生質柴油及生質丁醇作為生質燃料,並產出大豆胚芽粉及麩胱甘肽酵母菌粉作為生技保健產品之主原料,以提升整體附加價值降低總生產成本。未來將持續進行製程放大與最適化製程之探討,並整合其他生質能源生物製程,期能更符合達成產業經濟發展、社會經濟效益與永續發展的目標。 |
英文摘要 | The purpose of this study is to develop production methods for biomass alcohol, biomass butanol, biodiesel, glutathione, and soy germ powder using integrated bioprocesses. The feed sources are cassava starch and soybean. Bioethanol and glutathione (GSH) could be produced using cassava starch for fusion yeast fermentation by sequentially fed-batch fermentation. The fermentation production of bioethanol (for 46 h) and GSH (produced sequentially for 36 h) were 12.0 wt% and 19.4 mg/g-DCW, respectively. The cassava starch alcohol residues can be reused to feed the Cl. acetobutylicum S1 anaerobic fermentation, and up to 3.1 g/L of biobutanol was produced (0.40 mol butanol/mol sugar). The separation of soybean hypocotyls from the soybean feed source was obtained by centrifugation - bombardment (1.66% recovery rate). The composition of this product is rich in soy isoflavones and various physiologically active substances that can be used for raw biomaterials of health food. The remaining soybean cotyledon was treated using hexane to extract soybean oil (15.1% extraction rate). This crude soybean oil and the biobutanol can be used as a feedstock for the immobilized R. oryzae BD5, and biodiesel could be produced by transesterification. The optimum process conditions are as follows: a molar ratio of biobutanol/soybean oil is 3, 4 wt.% immobilized microorganism content, 5 wt% moisture content, and a 72 h reaction time. The bioconversion rate was 78.84%. The immobilized R. oryzae BD5 microorganism could be used consecutively for 4 times and still retain 80% activity. The integrated bioprocesses discussed in this study could be used to produce bioethanol, biodiesel, and biobutanol as biofuels. The soy germ powder and glutathione-contained yeast powder outputs could be used as raw biomaterials of health food. These processes could enhance the overall value added and reduce the prime cost of production. However, the scale-up process and optimal conditions require further investigations. These processes could also be integrated with other bioenergy processes to achieve industrial advancements, economic benefits, and sustainable developments. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。