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| 題 名 | Phylogenetic Analysis and Isolation of Canine Distemper Viruses in Taiwan=臺灣地區犬瘟熱病毒分離及血球凝集素基因分析 |
|---|---|
| 作 者 | 梁鍾鼎; 闕玲玲; 李泔泓; 黃宣憲; 梁善居; 余俊強; 劉振軒; | 書刊名 | 臺灣獸醫學雜誌 |
| 卷 期 | 34:4 2008.12[民97.12] |
| 頁 次 | 頁198-210 |
| 分類號 | 437.246 |
| 關鍵詞 | 犬瘟熱病毒; H基因; 臺灣; Canine distemper virus; Hemagglutinin gene; Taiwan; |
| 語 文 | 英文(English) |
| 中文摘要 | 於2003-2005年間自17隻未經疫苗注射之發病幼犬,應用患犬血液單核球與B95a細胞株共同培養之技術,分離出兩株具誘發融合細胞病變之病毒,經免疫螢光染色及抗原測試確認為犬瘟熱病毒。將此兩株病毒之血球凝集素基因(H),與同期間自台灣大學動物醫院臨床送檢病例之另外4株犬瘟熱病毒之H基因進行核酸定序。經比對序列與樹狀圖分析發現,本土病毒株皆有9個N連結配醣位,其中第7個配醣位為日本或中國大陸流行之亞洲1型犬瘟熱病毒所特有。本研究顯示臺灣地區所流行之犬瘟熱病毒,經其H基因分析屬亞洲1型。 |
| 英文摘要 | During the period from 2003 to 2005, two canine distemper virus (CDV) strains were isolated from 17 non-vaccinated puppies with suspected canine distemper by co-culture of peripheral blood mononuclear leucocytes and B95a cells. In addition, four cloned hemagglutinin (H) genes were obtained from 166 dogs infected with CDV. Indirect immunofluorescence assays and antigen tests confirmed that they were CDV. Analysis of the H genes of the six identified strains revealed that the deduced amino acid sequences contained nine potential sites for N-linked glycosylation, as had been found for H proteins of Japanese isolates. The seventh site is characteristic of the Taiwan strains described in this report and of recently reported Japanese strains. Furthermore, phylogenetic analysis of the H gene showed that the six isolates belong to the Asia-i group and are closely related to the recently reported Japanese and Chinese strains. |
本系統中英文摘要資訊取自各篇刊載內容。