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題名 | A Repetitive Sequence Specific to Oryza Species with BB Genome and Abundant in Oryza Punctata Kotschy ex Steud=野生水稻物種中B型基因組特有的重複序列之研究 |
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作者 | 鄭月雲; 方紹安; 林耀正; 鍾美珠; Cheng, Yueh-yun; Fang, Shao-an; Lin, Yao-cheng; Chung, Mei-chu; |
期刊 | Botanical Studies |
出版日期 | 20070700 |
卷期 | 48:3 2007.07[民96.07] |
頁次 | 頁263-272 |
分類號 | 434.114 |
語文 | eng |
關鍵詞 | 螢光原位雜合反應; 野生稻; 重複序列; 物種專一性; 逢機引子多型性標誌; Fluorescent in situ hybridization; FISH; Oryza punctata; Repetitive sequence; Species-specific RAPD; |
中文摘要 | 利用分子標記可以辨認特定的基因組及監測種間雜交後代之基因組滲入程度。本文報導由野生稻Oryza punctata Kotschy ex Steud (W1593)分離得的逢機引子擴增DNA片段(RAPD),命名為Opum210,此為稻屬物種中帶有B型基因組者所特有的重複序列。以逢機引子UBC210對稻屬各物種進行之聚合?增富反應,增幅所得產物中有一個B型基因組專一性的條帶出現,稱之為Opun210。本研究選殖並分析由非洲野生水稻O. punctata (W1593)基因組DNA擴增所得的Opun210片段;Opun210是第一個被報導的稻屬B型基因組物種專一性的重複序列,具有高度的物種專一性,甚至可以有效的分辨同一物種之不同族群。Opun210片段長度為789bp,在O. puntata (W1593)單套基因組內約有5.3×□個重複。將Opun210序列與基因資料庫中的已知序列比對,可找到少數的同源序列,且在5’端約有500bp長度的核?序列的同源性較高,而在3’端核?序列的同源性較低。SCAR-PCR的結果確定Opun210序列為帶有B型基因組之稻屬物種特有。此外,在Opun210序列中,位在第430~480的核?序列與一個Ty3-gypsy型的反轉錄跳躍子之蛋白的預測編碼區有88%相同,或與一個未知蛋白的編碼有94%相同。南方墨漬分析及螢光原位雜合反應的結果顯示,高度重複的Opun210序列散布在O. puntata全基因組內。根據所得到的資料,本文討論Opun210序列的起源與其在稻屬各物種間的歧異性。 |
英文摘要 | Molecular markers are capable of discriminating specific constituents of genome and monitoring genomic introgression of interspecifc hybrids. In this study, we isolate and characterize a BB genome specific RAPD, Opun210, from Oryza punctata Kotschy ex Steud (W1593), an African native wild rice. We demonstrate the Opum 210 as a highly species-specific marker. The Opum210 sequence is 789 base paris in length and estimated at 5.3 × □ copies in O. puntata (W1593) haploid genome, which contains the most repeats of OPUM210 among Oryza species. The results of DNA sequence alignments among Opun 210 most a few hits in the GenBank found that a relatively high similarity was in position~500 nucleotides regions at the 5’ ends, but a low similarity was in the rest of the nucleotides at the 3’ ends. SCAR-PCR profiles indicates that this fragment was specific to BB genome. Futhermore, the Opun210 sequence at position 430~480 nucleotides putatively encodes a peptide with 88% identiy to a Ty3-gypsy retrotransposon protein or a peptide with 94% identity to a hypothetical protein. The results of Southern hybridization and flurescent in situ hybridization (FISH) indicated that the repetitive Opum210 sequences dispersed throughtout the entire genome of O. punctata. The origin and divergence of the Opun210 sequence in genus Oryza is discussed based on the investigations in this study. |
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