頁籤選單縮合
題 名 | Microbial Contamination of the Tzu-Chi Cord Blood Bank from 2005 to 2006=2005至2006年慈濟臍帶血庫之微生物污染 |
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作 者 | 陳淑惠; 鄭雅君; 楊尚憲; 楊國樑; 石明煌; 何愉懷; | 書刊名 | 臺灣兒科醫學會雜誌 |
卷 期 | 49:1 2008.01-02[民97.01-02] |
頁 次 | 頁9-13+42 |
分類號 | 414.7 |
關鍵詞 | 臍帶血; 微生物污染; 造血先驅細胞; Cord blood; Microbial contamination; Hematopoietic progenitor cells; |
語 文 | 英文(English) |
中文摘要 | 背景:2005至2006年2年間,佛教慈濟幹細胞中心共收集4502袋臍帶血。此研究之目的爲瞭解臍帶血之微生物污染率以及菌種,另外也討論遭細菌污染之造血先驅細胞移植物對臨床預後之影響。方法:臍帶血送達實驗室後採集檢體做第一次的微生物培養,接著臍帶血經過一些處理,於冷凍前再採集檢體送一次微生物培養。此研究目的即是分析這些細菌培養結果。結果:慈濟臍帶血庫之微生物污染率爲1.8% (82/4502),致病菌佔所有污染微生物9.4% (8/85),3袋臍帶血長出2種細菌,Escherichia coli Bacteroid fragilis,Lactobacillus spp.,Enterococcus,β-Streptococcus Group B,Bacteroides valgatus,Corynebacterium spp.,Klebsiella pneumoniae和Peptococcus spp.,爲最常見之菌種,剖腹產的臍帶血微生物污染率低於陰道自然產(0.85%相對於2.16%)。結論:對於臍帶血的收集、處理及執行計畫,若有好的訓練可減少微生物污染率,密閉系統收集、子宮外收集方法有較低的微生物污染率,我們的臍帶血庫採用密閉系統收集但使用子宮內收集方法;此研究報告我們臍帶血庫的成續,如同其他研究報告,大部分的微生物爲非致病性的。 |
英文摘要 | Background: In total, 4502 units of cord blood (CB) were collected during a 2-year period from 2005 to 2006 by the Buddhist Tzu-Chi Stem Cells Center. The aim of this study was to analyze the incidence of microbial contamination and type of organism present in the cord blood. The clinical impact of microbial contamination on hematopoietic progenitor cell (HPC) grafts used for HPC transplantation is also discussed. Methods: First and second specimens were obtained for microbial assessment. These were collected in laboratory after cord blood collection and after cord blood unit manipulation, respectively. The samples were cultured and the results reviewed. Results: The overall incidence of microbiological contamination was 1.8% (82/4502).Three CB units were contaminated with two different organisms. Infectious organisms comprised 9.4%(8/85) of total isolated microbes. These infectious microorganisms were β-Streptococci group B, Candida tropicalis and Staphylococcus aureus which were isolated in 6, 1 and 1 of CB units respectively. Escherichia coli, Bacteroides fragilis, Lactobacillus spp., Enterococcus, β-Streptococcus Group B, Bacteroides valgatus, Corynebacterium spp., Klebsiella pneumonia and Peptococcus spp. were the most frequently encountered microorganisms. A higher contamination rate of the CB units was noted after vaginal delivery (2.16%) compared to caesarian section (0.85%) (p<0.01). Conclusions: Extensive training in CB collection, good procedures and good protocols can decrease the rate of microbial contamination. The use of a closed collecting system and an ex utero method have the advantage of a lower contamination rate. In our cord blood bank, we use a closed system but an in utero method. Similar to other studies, most of microorganisms reported here as contaminants are non-pathogenic. |
本系統中英文摘要資訊取自各篇刊載內容。