頁籤選單縮合
題名 | Human Umbilical Cord Blood Cells Protect Against Hypothalamic Apoptosis and Systemic Inflammation Response during Heatstroke in Rats= |
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作者 | Liu, Won-shiung; Chen, Chun-ta; Foo, Ning-hui; Huang, Hsuan-rong; Wang, Jhi-joung; Chen, Sheng-hsien; Chen, Te-jen; |
期刊 | Pediatrics and Neonatology |
出版日期 | 20091000 |
卷期 | 50:5 2009.10[民98.10] |
頁次 | 頁208-216 |
分類號 | 414.7 |
語文 | eng |
關鍵詞 | Cytokines; Heatstroke; Human umbilical cord blood cells; Inflammation; Rat; |
英文摘要 | Background : Intravenous administration of human umbilical cord blood cells (HUCBC) has been shown to improve heatstroke by reducing arterial hypotension as well as cerebral ischemia and damage in a rat model. To extend these findings, we assessed both hypothalamic neuronal apoptosis and systemic inflammatory responses in the presence of HUCBCs or vehicle medium immediately after initiation of heatstroke. Methods : Anesthetized rats, immediately after the initiation of heat stress, were divided into two groups and given either serum-free lymphocyte medium (0.3 mL per rat, intravenously) or HUCBCs (5× 106 in 0.3 mL serum-free lymphocyte medium, intravenously). Another group of rats were exposed to room temperature (26ºC) and used as normothermic controls. Heatstroke was induced by exposing the anesthetized rats to a high ambient temperature of 43ºC for 68 minutes. Results : After the onset of heatstroke, animals treated with serum-free lymphocyte medium displayed hyperthermia, hypotension, bradycardia, hypothalamic neuronal apoptosis and degeneration, and up-regulation of systemic inflammatory response molecules including serum tumor necrosis factor-alpha, soluble intercellular adhesion molecule-1 and E-selectin. Heatstroke-induced hypotension, bradycardia, hypothalamic neuronal apoptosis and degeneration, and increased systemic inflammatory response molecules were significantly inhibited by HUCBC treatment. Although heatstroke-induced hyperthermia was not affected by HUCBC treatment, the serum levels of the anti-inflammatory cytokine interleukin-10 were significantly increased by HUCBC therapy during hyperthermia. Conclusions : These findings suggest that HUCBC transplantation may prevent the occurrence of heatstroke by reducing hypothalamic neuronal damage and the systemic inflammatory responses. |
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