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題名 | Antiproliferative Effect of Different Cultivated Antrodia Cinnamomea on Hep G2, Hep 3B and PLC/PRF/5 Hepatoma Cells=不同培養樟芝(Anthodia Cinnamomea)對Hep G2、Hep 3B和PLC/PRF/5肝癌細胞的抗增生效應 |
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作者 | 黃良得; 林俊清; 許雅玲; 郭悅雄; 賴敏男; | 書刊名 | 大仁學報 |
卷期 | 31 2007.09[民96.09] |
頁次 | 頁69-78 |
分類號 | 414.5 |
關鍵詞 | 樟芝; 抑制增生; Antrodia cinnamomea; Antiproliferative; Hep G2; Hep 3B; PLC/PRF/5; |
語文 | 英文(English) |
中文摘要 | 本研究目的為探討8種不同品系的培養樟芝(Anthodia Cinnamomea;AC)子實體(如AC-1、AC-2、AC-3、AC-4、AC-5、AC-6、AC-7和AC-8),2種樟芝菌絲體(代號為35396與35398)和一種市售樟芝菌絲體產品(ACM)的萃取物之抗肝癌活性。實驗使用XTT方法對Hep G2、Hep 3B和PLC/PRF/5三種肝癌細胞株進行分析。結果顯示樟芝水萃取物(ACE)和水級分部份具有微弱的抗肝癌活性,此效力與樟芝品系及種類無關。在三個不同級分部份[水(H₂O)、乙酸乙酯(EA)和正丁醇(BuOH)]之間,EA級分呈現最佳的活性;對Hep G2細胞具有最佳活性的萃取物是AC-2 EA(IC[9274]=42.6 µg/ml),對Hep 3B細胞是AC-4 EA(IC[9274]=64.9 µg/ml),和對PLC/PRF/5細胞是AC-7 EA(IC[9274]=40.6 µg/ml)。除了EA部份外,其他菌絲體的級分萃取物對所有實驗細胞株皆具微弱之抑制細胞增生效果(IC[9274]>250 µg/ml)。在正丁醇部份,只有AC-2對PLC/PRF/5細胞具有適中的活性(IC[9274]=134.5 µg/ml)。本研究結論說明樟芝的抗肝癌活性會隨品系和所使用溶劑製備的萃取物而改變;此結果也斷定具有潛力的抗肝癌成分是存在於乙酸乙酯級分部份。 |
英文摘要 | This study aimed to examine the antiphepatoma activity of extracts prepared from fruiting bodies of eight different cultivated Antrodia cinnamomea (AC) accessions, namely AC-1, AC-2, AC-3, AC-4, AC-5, AC-6, AC-7 and AC8, two AC mycelia namely 35396 and 35398, and a commercial AC mycelial product (ACM). Using XTT assay, three human hepatoma cells, namely Hep G2, Hep 3B and PLC/PRF/5 were tested. Results showed that both aqueous extract (ACE) and H₂O fraction of AC, irrespective of accessions and fungal components, showed, a weak antihepatoma activity. Among the three different fractions [water (H₂O), ethylacetate (EA) and n-butanol (BuOH)], EA fraction demonstrated the greatest potency, the most active extract for Hep G2 was AC-2 EA(IC□= 42.6μg/ml), for Hep 3B was AC-4 EA (IC□= 64.9μg/ml) and for PLC/PRF/5 was AC-7 EA (IC□= 40.6μg/ml). With the exception of EA fraction, other mycelial fractions showed a weak antiproliferative effect on all cell lines tested (IC□ > 250μg/ml). For the BuOH fraction, only AC-2 showed a moderate activity on PLC/PRF/5 (IC□= 134.5μg/ml) cells. The present study concludes that the antihepatoma activity of AC varies with the type of accessions and solvent used to prepare the extract. The results also suggest that the potent antihepatoma constituents could be present in the EA fraction. |
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