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| 題 名 | Effect of Sulfite-treated Daylily (Hemerocallis Fulva L.) Flower on the Production of Nitric Oxide and DNA Damage in Macrophages=亞硫酸鹽處理金針花對巨噬細胞生成一氧化氮及DNA損傷之影響 |
|---|---|
| 作 者 | 陳惠英; 薄榮怡; 黃雯華; 顏國欽; | 書刊名 | 藥物食品分析 |
| 卷 期 | 15:1 2007.03[民96.03] |
| 頁 次 | 頁63-70+96 |
| 分類號 | 412.37 |
| 關鍵詞 | 金針花; 一氧化氮; 二氧化硫; DNA損傷; Daylily flower; Hemerocallis fulva L.; Nitric oxide; Sulfur dioxide; DNA damage; |
| 語 文 | 英文(English) |
| 中文摘要 | 本研究為探討不同來源之新鮮金針花與乾燥金針花抑制一氧化氮生成的能力,以及評估二氧化硫的存在是否會影響乾燥金針花抑制一氧化氮的生成及細胞DNA損傷方面的表現。結果顯示,亞硫酸鹽處理及未處理之乾燥金針花清除sodium nitroprusside (SNP)生成一氧化氮的能力較佳,而新鮮金針花則較差。另外新鮮金針花與未經亞硫酸鹽處理之乾燥金針花抑制lipopolysaccharide (LPS)誘導巨噬細胞生成一氧化氮的能力較佳,而經亞硫酸鹽處理之乾燥金針花則較差。進一步測定其二氧化硫含量,並換算成相對濃度之亞硫酸氫鈉(NaHSO₃),來探討NaHSO₃抑制一氧化氮生成及細胞基因傷害的能力。結果顯示,隨著亞硫酸氫鈉濃度的增加,其清除SNP生成一氧化氮的能力隨之增加,且其亦有抑制LPS誘導細胞生成一氧化氮的能力,然抑制能力不高。此外,NaHSO₃亦會稍微引起細胞DNA損傷。雖然在加熱時二氧化硫會揮發掉,但仍有二氧化硫殘留之虞,因此對於乾燥金針花的攝取仍以適量為宜。 |
| 英文摘要 | The inhibitory effect of fresh or dried daylilies extract on the generation of nitric oxide, and the effects of sulfur dioxide in dried daylily on nitric oxide production and DNA damage in Raw 264.7 macrophages were examined. The extracts from sulfite-treated and untreated dried daylily flowers exhibited stronger scavenging effects on nitric oxide production by sodium nitroprusside (SNP) than that of fresh flower. Fresh and untreated dried daylily flowers exhibited strong inhibitory effect on nitric oxide induced by lipopolysaccharide (LPS) in macrophages, but the sulfite-treated dried daylily did not. The scavenging effect of sulfite on nitric oxide production by SNP was dose-dependent, and sulfite also exhibited low inhibitory effect on nitric oxide induced by LPS in macrophages. Sodium hydrogen sulfite also slightly induced DNA damage in macrophages. Although sulfur dioxide in dried flowers is volatilized during heating, its residual adverse effects should still be concerned. |
本系統中英文摘要資訊取自各篇刊載內容。