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題名 | Isolation and Analysis of a Novel Proteoglycan from Zizyphus Jujuba cv. Jinsixiaozao=金絲小棗蛋白聚糖的分離及其結構分析 |
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作者 | 李進偉; 陳義勇; 丁紹東; 張連富; Li, Jin-wei; Chen, Yi-yong; Ding, Shao-dong; Zhang, Lian-fu; |
期刊 | 藥物食品分析 |
出版日期 | 20070900 |
卷期 | 15:3 2007.09[民96.09] |
頁次 | 頁271-277+335 |
分類號 | 412.37 |
語文 | eng |
關鍵詞 | 金絲小棗; 蛋白聚糖; 紅外光譜; β-消去反應; 抗補體活性; Zizyphus jujuba cv; Jinsixiaozao; Proteoglycan; Fourier transforms infrared spectra; β-elimination reaction; Anti-complement activity; |
中文摘要 | 紅棗作為藥食同源物,具有許多生理活性。本文對金絲小棗蛋白聚糖的提取純化方法、結構以及它對補體系統的影響進行了研究。以棗果為材料,經熱水抽提、乙醇沉澱、陰離子交換管柱層析和凝膠管柱層析,分離純化得白色粉末狀棗蛋白聚糖(ZSG4b)。ZSG4b經高效凝膠滲透層析和凝膠管柱層析鑑定為均一組分,測得其分子量為1.4 × □Da。化學組成分析顯示ZSG4b含83.5%多糖,9.7%蛋白質。氣相層析顯示ZSG4b含鼠李糖、阿拉伯糖、甘露糖、半乳糖、其莫耳比為13.8:4:3:8;胺基酸組成分析顯示ZSG4b幅含天冬胺酸、麩胺酸、絲胺酸、蘇胺酸和白胺酸,這五種胺酸占總胺基酸莫耳百分比的62.8。β-消去反應顯示ZSG4b中的多糖與蛋白質係由O-型糖?鍵結合。ZSG4b在體外有顯的抗補體活性,活性隨其濃度的增大而增加。 |
英文摘要 | Chinese jujube (Zizyphus jujube Miller) is a well-known oriental medicinal plant that has various biological activities. In this study, a novel water-soluble proteoglycan (ZSG4b) was isolated from Zizyphus cv. Jinsixiaozao and its effect on the complement system was investigated. ZSG4b was extracted with hot water and purified by DEAE-Sepharose CL-6B and Sepharose CL-6B column chromatography. It was eluted as a single symmetrical narrow peak on high-performance gel-permeation chro-matography (HPGPC) and the average molecular weight was estimated to be 1.4 × □ Da. ZSG4b contained 83.5 glycan and 9.7% protein. Gas chromatography analysis result indicated that the glycan portion of ZSG4b was composed mainly of rhammose, arabionse, mannose and galactose in a molar ration of 13.8:4:3:8. It also contains 29% uronic acid as determined by m-hydroxy-biphenyl method. The presence of galacturonic acid was estimated by the increase of galactose content in the carboxyl-reduced ZSG4b. The amino acid composition showed that it was rich in aspartic acid, glutamic acid, serine, threonine and leucine; the molar content of these five amion acids constituted 62.8% of the total amino acids. The β-elimination reaction demonstrated that the protein and glycan were linked by O0linkage. ZSG4b exhibited high anti-complement activity which was increased in accordance with the concentration below 120 μg/mL in vitro. |
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