頁籤選單縮合
題名 | 反芻動物飼料試管乾物質消化率(IVDMD)方法之修改=Protocol Modification of in Vitro Dry Matter Digestibility Methods for Ruminant Feed Evaluation |
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作者 | 李春芳; 蕭宗法; | 書刊名 | 畜產研究 |
卷期 | 40:1 2007.03[民96.03] |
頁次 | 頁59-65 |
分類號 | 437.11 |
關鍵詞 | 試管乾物質消化率; 消化時間; 胃蛋白酶濃度; Digestion period; In vitro dry matter digestibility; Pepsin level; |
語文 | 中文(Chinese) |
中文摘要 | 兩段式試管乾物質消化率(in vitro dry matter digestibility, IVDMD),是一項公認與反芻動物飼料活體消化率相關高的實驗室方法,其包括48 h瘤胃微生物發酵與48 h胃蛋白酶消化,但一批次測定會跨越兩週時間,因此本試驗目的在探討如何修改第二階段胃蛋白酶的消化流程,以提升營養評估的工作效率。收集牧草、副產物及精料三類飼料進行試驗。試驗一比較胃蛋白酶消化時間之影響,包括24 h、30 h及48 h(對照組)三處理,得知縮短消化時間會顯著降低消化率(P = 0.02)。試驗二在胃蛋白酶消化24 h設定下,比較胃蛋白酶濃度之影響,得知胃蛋白酶1倍(5%)的消化率與對照組相近,而2倍濃度(10%)有提升消化率之趨勢(P = 0.06)。試驗三再增加胃蛋白酶濃度1.2倍(6%)處理,四處理組48 h + 48 h (5%,對照組)、48 + 24 (5%)、48+24 (6%)及48+24 (10%)之IVDMD相近,依序為62.9%、62.8%、63.2%與63.4%。綜合試驗結果建議,IVDMD第二階段之消化時間可以修改為24 h,並使用6%酵素濃度。兩項修改可以達到一週(五個工作天)完成一批次完整測定的目標,顯著提升IVDMD營養評估工作效率達50%。 |
英文摘要 | In vitro dry matter digestibility (IVDMD) is a widely recognized laboratory method that highly correlated with in vivo digestion. It consists of the first 48-h rumen microbial fermentation and the second 48-h pepsin digestion. Due to the 2-wk length for each complete batch run, it was aimed to modify the pepsin digestion procedure to promote its efficiency in terms of feed nutrition evaluation. Three categories of feed, forages, byproducts and grain concentrates, were assayed in this study. In trial I, pepsin digestion period of 24 h, 30 h and 48 h (control) were compared. Simply shortening the period of pepsin digestion would significantly decrease their IVDMD values (P = 0.02). In trial II, with 24-h pepsin digestion, effect of increasing pepsin level was studied. It showed that digestibility from 2-fold pepsin level (10%) treatment tended to be higher than control group (P = 0.06). In trial III, IVDMD values were similar among four treatments, 48+48 (5% pepsin, control), 48+24 (5%), 48+24 (6%) and 48+24 (10%), and averaged 63.08%. It was suggested that the second stage of IVDMD method could be modified as 24 h in length with 6% of pepsin level. These two modifications make it possible to accomplish one batch IVDMD measurement within one week and thus significantly improve the evaluation efficiency by 50%. |
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