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題名 | Solid-phase Extraction and High-performance Liquid Chromatographic Analysis of Prednisone Adulterated in a Foreign Herbal Medicine=中草藥中摻加Prednisone之固相萃取與高效液相層析定量分析 |
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作者 | 顧祐瑞; 劉宜祝; 林哲輝; Ku, Yoe-ray; Liu, Yi-chu; Lin, Jer-huei; |
期刊 | 藥物食品分析 |
出版日期 | 20010900 |
卷期 | 9:3 2001.09[民90.09] |
頁次 | 頁150-152 |
分類號 | 414.33 |
語文 | eng |
關鍵詞 | 固相萃取; 高效液相層析; 中藥製劑; 中藥摻加西藥之檢驗; HPLC; Prednisone; Solid-phase extraction; Herbal medicine; Adulterant; |
中文摘要 | 一種來自東南亞的中草藥檢體,以薄層層析及紫外分光光度計檢驗出含 prednisone 成分,經固相萃取管處理後,再以高效液相層析法定量。 固相萃取管依序以甲 醇及氯仿先活化,繼用二氯甲烷及異丙醇 (6:4, v ╱ v) 沖提檢體。高效液相層析採用逆 相層析管注 (Inertsil ODS-80A),沖提液為乙 與水 (3:7,v ╱ v),檢測波長為 240nm ,內部標準品為 fludrocortisone acetate。Prednisone 檢量線之濃度範圍為 10.0-200.0 μ g/mL。 確效結果顯示精密度、準確度及回收率均良好。以上述方法定量分析檢出每包檢 體 (約 21 公克 ) 含 prednisone 5.5 毫克。 |
英文摘要 | Prednisone was detected in a herbal medicine from Southeast Asia using thin layer chromatography (TLC) and ultraviolet spectroscopy (UV). After treating with solid-phase extraction (SPE), the sample was further assayed by high-performance liquid chromatography (HPLC). The sample solution was achieved with a silica gel cartridge conditioned with methanol and chloroform in sequence, and then eluted with dichloromethane-isopropanol (6: 4, v/v) . Separation was conducted with an Inertsill ODS-80A reversed-phase column using isocratic elution with acetonitrile and water (3:7, v/v) as mobile phase. Fludrocortisone acetate was used as an internal standard and detection wavelength was at 240 nm. Calibration curve of standard prednisone was constructed in the range of 10.0-200.0 μ g/mL. The content of adulterated prednisone was 5.5 mg/21g sample. |
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