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題名 | Validated Method for the Determination of Arbutin in Whitening Cosmetics by High-Performance Liquid Chromatography Coupled with On-Line Microdialysis Sampling=微透析取樣連線結合高效液相層析測定美白化妝品中之熊果素的方法確認 |
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作者 | 林正輝; Lin, Cheng-hui; |
期刊 | 永達學報 |
出版日期 | 20060200 |
卷期 | 6:2 民95.02 |
頁次 | 頁34-45 |
分類號 | 412.36 |
語文 | eng |
關鍵詞 | 微波析取樣; 高效液相層析法; 熊果素; 美白化妝品; 確認; Microdialysis sampling; High-performance liquid chromatography; Arbutin; Whitening cosmetics; Validation; |
中文摘要 | 利用微透析取樣結合高效液相層析已被研發用來測定美白化妝品中之熊果素。以去離子水當灌流液、微透析取樣膜長度為10毫米及灌流液流速每分鐘5微升可獲得最佳之分析條件。將熊果素標準液加入化妝品空白乳液中進行同一天及不同天之準確度與精密度之分析。準確度(以偏差表示)同一天(重複分析六次)及不同天(連續五天,共30次)之範圍從-8.9至11.5%,精密度低於7.64%相對標準偏差。校正曲線之線性範圍從0.1mM至20mM(線性回歸之R平方值為0.9989)。從訊號雜訊比值為3倍所得到之偵測極限值為15μM。本連線系統分析方法與定量分析常使用之no net flux (NNF)法進行美白化妝品中熊果素測定結果之比對,顯示本研發之微透析取樣結合高效液相層析連線系統具有高準確度。以Plackett-Burman實驗設計評估本分析方法之剛硬性(rubustness),除了低灌流速會造成透析回收率增加12.52±2.31%外,其他參數並不會顯著的影響分析結果。本研發方法具有簡易樣品前處理、高可靠度、快速分離及低有機溶劑消耗之優點,故適合在同一天進行大量樣品之例行分析。 |
英文摘要 | We have developed a method that uses on-line microdialysis sampling coupled with high-performance liquid chromatography (HPLC) to determine arbutin in whitening cosmetics. When using deionized water as the perfusate, we found that the optimum conditions for the dialysis during microdialysis sampling were a probe length of 10 mm and a dialysis flow-rate of 5 μl min¯¹. Adding arbutin standards to a cosmetic cream that did not contain arbutin allowed us to monitor the intra- and inter-day accuracy and precision of our analysis. The accuracy (% bias) for intra-day (n=6) and inter-day (n=30, five consecutive days) analyses ranged from -8.9 to 11.5%, with a precision below 7.64% RSD. The calibration curve was linear within the range from 0.1 to 10 mM (R²=0.9989). The detection limit-defined as three times the signal-to-noise ratio--was 15 μM. By comparing the arbutin levels etermined this way in the whitening cosmetics with the results obtained from the no net flux method, we conclude that our proposed on-line microdialysis-HPLC system displays good accuracy. We evaluated the robustness of our optimum conditions by means of a Plackett-Burman design. Apart from the effect of a low flow-rate of perfusate--an increase of 12.52±2.31%--we observed no significant changes in the analyses upon changing the levels of any other parameter. Because this method offers the advantages of simplicity, reliability, rapidity, the lack of any tedious sample pretreatment process and a reduced use of organic solvents, we believe that is is suitable for the routine analyses of a large number of samples within the same day. |
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