頁籤選單縮合
題名 | Virtual Microchannels for Focusing and Guiding Fluorescent Microbeads Using Dielectrophoretic Force=運用介電泳法聚焦與導引微粒子之虛擬微流道功能 |
---|---|
作者 | 莊承鑫; 朱敬威; 黃耀緯; Chuang, Cheng-hsin; Ju, Jing-wei; Huang, Yao-wei; |
期刊 | 南臺學報 |
出版日期 | 20120900 |
卷期 | 37:3 2012.09[民101.09] |
頁次 | 頁67-74 |
分類號 | 448.552 |
語文 | eng |
關鍵詞 | 介電泳; 虛擬微流道; 螢光粒子; Dielectrophoresis; DEP; Virtual microchannel; Fluorescent microbead; |
中文摘要 | 本研究完成一可程式化介電泳晶片,利用晶片內之電極設計可快速的達到聚焦與導引粒子之功能,不需要複雜的流道設計即可完成粒子之操控,此方法稱之為虛擬微流道。然而,欲了解虛擬微流道的導引率之多寡,因此,將以不同流速與導引角度去得到虛擬微流道的最佳設計參數。晶片主要包含二大部分,其一為上層ITO導電玻璃,其二為運用黃光微影製程技術製作底層電極,雙層電極間以OCA光學雙面膠(Optical Clean Adhesive)做為微流道腔體,不僅可以節省光阻之成本,也可省去製作腔體所花費的時間與製程步驟。且底層主要分為二大部份:其一為魚骨頭聚焦電極,以負介電泳力驅動使粒子聚焦;其二為導引電極,利用不同的開關控制不同角度之導引電極,使粒子可依照電極角度與形狀導引至標的區域。根據模擬與實驗結果顯示,粒子可以成功的聚焦與導引至標的區域於本介電泳晶片,且利用不同角度之導引電極 (15°, 30° 與 45°)與螢光粒子可評估粒子導引率之多寡。模擬與實驗結果證實此介電泳晶片具有粒子聚焦與導引雙功能,未來更可將此功能運用於奈米粒子操控與生物醫學檢測之研究。 |
英文摘要 | In this study a programmable dielectrophoretic chip is developed, whose electrode design can be used to rapidly focus and guide microbeads, without the need of a complicated channel design to manipulate the microbeads. The method used for this chip is called ‘virtual microchannel’. The guiding ratio of microbeads depends on two important parameters: the expanding angle of the bottom electrode and the flow rate. Therefore, a series of studies on these two important parameters are conducted to achieve the optimal virtual microchannel design. The structure of a the DEP chip consists of a top electrode made of indium tin oxide (ITO), a flow chamber formed by optically clear adhesive (OCA) tape, and two kinds of bottom electrodes on a glass substrate. The bottom electrodes can be divided into two parts: the first part is for focusing the suspended microbeads by a fish-bone type electrode operated in a negative DEP range; the second part is for switching and guiding these focused microbeads along the electrode surface to the target area, like a flow passing along a virtual channel. The results of the simulation and experiment indicate that microbeads can be aligned along the gap of the fish-bone electrode and be successfully guided into the target area at various angles (15°, 30° and 45°) at a low flow rate. In the future, the virtual microchannel could be further employed for the research of nanoparticle manipulation and biomedical application. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。