查詢結果分析
來源資料
相關文獻
- Examination of Meat Components in Commercial Dog and Cat Feed by Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphisme (PCR-RFLPs) Technique
- 寵物飼料污染三聚氰胺及三聚氰酸爆發犬貓腎衰竭事件之回顧
- 醋氨酚(acetaminophen)中毒
- 從營養角度探討犬貓繁殖與初生仔的健康
- 犬貓呼吸器及心臟血管系統疾病
- 犬貓常見的皮膚病
- 抗氧化劑對健康寵物與生病寵物的不同功效
- 照顧高齡寵物:老年化小動物的不同營養需求
- 臺灣白鼻貍貓人工感染假性狂犬病病毒之檢測
- Capillary PCR for Detecting and Differentiating Feline Panleukopenia Virus and Canine Parvovirus in Fecal Specimens
頁籤選單縮合
題 名 | Examination of Meat Components in Commercial Dog and Cat Feed by Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphisme (PCR-RFLPs) Technique=應用聚合酶連鎖反應-限制酵素切割圖譜(PCR-RFLPs)檢測市面販售犬、貓飼料中肉品種類來源 |
---|---|
作 者 | 王咸祺; 李淑慧; 張天傑; 王孟亮; | 書刊名 | 行政院農業委員會家畜衛生試驗所研究報告 |
卷 期 | 39 民92 |
頁 次 | 頁75-80 |
分類號 | 437.111 |
關鍵詞 | 聚合酶連鎖反應-限制酵素切割圖譜; 犬; 貓; 飼料; 肉品種類; BSE; Cyt b gene; Dog and cat commercial diet; PCR; Polymorphism; |
語 文 | 英文(English) |
中文摘要 | 本實驗目的為利用聚合酶連鎖反應-限制酵素圖譜 (PCR-RFLPs) 檢測犬貓飼料中肉品種類來源。由於牛海綿狀腦病 (俗稱狂牛症))之類的神經性疾病可能經由被污染的食物所引起,因此檢測動物飼料中肉品來源,對於獸醫公共衛生而言極為重要的。本實驗的結果利用聚合酶連鎖反應技術將動物細胞內線體的部份細胞素(cytochrome) b基因之片段增幅,之後再以限制酵素Alu 1及Mbo 1切割這些PCR產物,進而達到區分不同種類動物肉品來源之目的。本實驗共檢測八種市面上所販售的犬飼料以及貓飼料,結果顯示所有市售貓飼料部分所檢查之結果,有八分之五的品牌中有雞肉來源的成分。 |
英文摘要 | It has been shown that certain slow neurological diseases such as bovine spongiform encephalopathy (also known as "mad cow" disease) could be transmitted through contaminated food intake by animals; therefore, the examination of meat components in commercial feeds is important for the control of the disease in public health. The combination of polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLPs) technique was applied to examine the meat components in dog and cat commercial feeds. The partial nucleotide sequence (359 bp) of animal mitochondrial cytochrome b (cytb, CYT) gene was amplified by PCR and then digested with restriction enzyme Alu 1 or Mbo 1. In this work, eight brands of commercial dog and cat feed available in Taiwan were examined. All brands of dog feeds that were tested contained meat from four different animals (cattle, pig, goat and ckicken). In cat feeds, the chicken meat was found in five out of eight brands. |
本系統中英文摘要資訊取自各篇刊載內容。