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題名 | 不同培養基測定總菌落數的比較=Evaluation of the Use of Different Culture Media for the Total Plate Colonycount |
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作者 | 林明瀅; 王復德; 王永衛; Lin, Ming-yin; Wang, Fu-der; Wong, Wang-wai; Kou,Benjamin IT; |
期刊 | 院內感染控制雜誌 |
出版日期 | 20011000 |
卷期 | 11:5 2001.10[民90.10] |
頁次 | 頁289-298 |
分類號 | 414.8 |
語文 | chi |
關鍵詞 | 環境微生物; 微生物培養方法; 總菌落數; Environment microorganisms; Culture method; Total plate count; |
中文摘要 | 總菌落數檢查之目的在於檢測樣本中生存之好氧及兼性厭氧異營細菌、酵母菌及黴菌等總微生物之數目。美國公共衛生學會建議用於水質微生物檢測的方法,為傾倒平板法,使用 (tryptone glucose extract agar;TGEA)培養基。本研究主要在探討使用 TGEA 與各臨床微生物實驗室常用之培養基,對不同的標準品管菌是否會造成菌落生長數量的差別。測試菌株:分別有金黃色葡萄球菌 (Staphylococcus aureus)、大腸桿菌(Escherichia soli) 、綠膿桿菌(Pseudomonas aeruginosa)共三種,每個標準菌分別重複進行十次;另外並以臨床常見檢體進行測試。使用培養基分別為 (TGEA、Mueller-Hinton II agar;M-H II)、(tryptone soy agar;TSA)、(nutrient aqar;NA)。所有培養之菌落數先以 log10 轉換,以TGEA 培養基的菌落數作為比較的基準,再進行成對t檢定 (pair-t test)、線性迴歸統計分析。不論何種標準晶管菌株,接種量為 1mL 於不同培養基經傾倒平板法培養 48 小時後,菌落數經 logl0 轉換後,TGEA 與M-H II 的一致度為0.93(95% CI=0.86-0.96),另外與 TSA、NA 分別為 0.96 (95% CI=0.92-0.98)、0.94(95% CI=0.88-0.97)。而環境監測檢體,呼吸治療裝置潮溼器用水,血液透析用水,奶水,果汁冷飲,於上述四種培養基的茵落數,經成對 t 檢定,亦皆無統計上差異,三項資料顯示微生物實驗室中常見的培養基 M-H II、TSA 、 NA 可適用於呼吸治療裝置潮溼器用水、血液透析用水、奶水、果汁冷飲的總菌落數測定。(感控雜誌2001;11:289-98) |
英文摘要 | Total plate colony count, or total plate counting (TPC), is a method employed to estimate the bacterial or fungal loads of drinking water, food samples, or other liquids used in the medical care of patients. The standard method of American Public Health Association for the TPC is to use the pour-plate method on tryptone glucose extract agar (TGEA). We studied the feasibility of replacing the TGEA with other culture media commonly used in the clinical microbiology laboratory. The same protocol was used to measure the recovery of Staphylococcus aureus, Escherichia coil, and Pseudomonas aeruginosa in Muelller-Hinton II agar (M-H II ), tryptone soy agar (TSA), and nutrient agar (NA). The results were compared with those of the TGEA that were performed simultaneously. Each medium was repeatedly tested ten times for each of the three organisms, and also tested with moisturizing solutions for ventilators (13 specimens), milk (10), cold drinks (5), and dialysis fluids(45). All 73 of these specimens were tested simultaneously in TGEA and M-H ~i , and 13 in TGEA and TSA or NA. The colony counts were first converted to log 10 values. Paired t test, linear regression analysis and the agreement of intra-class correlation coefficient (ri) were used to compare the results generated by 4 culture media. The study showed that M-H II , TSA, and NA all produced comparable results by the agreement test (ri=0.93,0.96,0.94, respectively). Our results suggest that M-H II, T~A, and NA may be suitable as the alternatives to TGEA for the TPC of dialysis fluids, milk, cold drinks, and the moisturizing solutions for ventilators. (Nosooom Infect Control d 2001:11:28g-98) |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。