查詢結果分析
來源資料
相關文獻
- KLK1基因啟動子-130G[fed7]多型性與帕金森氏症感受性的分析
- 指導照護者照顧居家帕金森氏症患者的護理經驗
- Subthalamic Surgery for Advanced Parkinson's Disease
- The Genetic Importance of Varied Quantitative HLA Expression in Influencing Disease Susceptibility
- 帕金森氏症的最新治療
- 精神病用藥副作用之探討
- 一位帕金森氏症病患出院準備服務
- Language Disorders in Parkinson's Disease
- Diagnostic Sensitivity of Parkinsonism
- A Historical Note on the Discovery of Naturally Occurring L-DOPA in Taiwan
頁籤選單縮合
題名 | KLK1基因啟動子-130G[fed7]多型性與帕金森氏症感受性的分析=Analysis of KLK1 Gene-130G[fed7] Polymorphism with the Risk of Parkinson's Disease |
---|---|
作者 | 黃淑宜; 莊蕙瑄; 陳瓊美; 吳逸如; 李桂楨; Huang, Shu-yi; Juang, Huei-shiuan; Chen, Chiung-mei; Wu, Yih-ru; Lee-Chen, Guey-jen; |
期刊 | BioFormosa |
出版日期 | 20050600 |
卷期 | 40:1 民94.06 |
頁次 | 頁9-15 |
分類號 | 415.83 |
語文 | chi |
關鍵詞 | -130G[fed7]多型性; 帕金森氏症; 疾病感受性; KLK1; -130G[fed7] polymorphism; Parkinson's disease; Disease susceptibility; |
中文摘要 | 帕金森氏症為一常見的神經退化性疾病,和遺傳及環境因素相關。KLK1為一serine protease,在人體包括大腦皮質、海馬迴等多處部位皆有表現。本研究的目的在探討KLK1基因啟動子-130G(下標 N)多型性與國人帕金森氏症感受性的相關性。-130G(下標 N)多型性基因型的分析方法包括螢光PCR產物的長度分析、單股核酸構形多型性/異雙股分析及DNA定序等。所研究樣品群包括208位帕金森氏症患者及95位正常人。結果共觀察到五種對偶基因:I (G9)、A (G10)、B (G2CG7)、H (G11)、K (Gl2)。多型性對偶基因的啟動子轉錄活性分析顯示,在人類胚胎腎細胞HEK-293及人類腦癌細胞IMR-32中,A、B、H對偶基因的轉錄活性並無顯著差異,但三者的轉錄活性均較K對偶基因顯著的好。進一步的依據含或不含K對偶基因的基因型,或K對偶基因頻率,進行X^2統計分析,結果顯示在正常人族群與帕金森氏症患者群間,並未呈現顯著差異。故推論KLK1基因啟動子多型性,雖然會影響其在人類胚胎腎細胞及腦癌細胞中的表現量,但其變異和國人帕金森氏症的感受性並沒有太大關連性。 |
英文摘要 | Parkinson's disease (PD) is a common neurodegenerative disorder involving several genetic and environmental components. Human tissue kallikrein (KLK1) is a serine protease expressed in both the cerebral cortex and hippocampus. In this study v e investigated the association of KLK1 gene -130G(subscript N) polymorphism with the risk of Parkinson's disease. The -130G(subscript N) polymorphism as analyzed by electrophoresis of fluorescenced PCR products in sequencing gels. single-strand conformation polymorphism (SSCP)/heteroduplex analysis and DNA sequencing. Two hundred and eight patients with PD and 95 normal controls ere examined. Five alleles ere identified in the KLK1 promoter: I (G9), A (G10), B (G2CG7), H (G11), and K (G12). A reporter construct containing the K allele cloned into a luciferase reporter plasmid drove significantly lower transcriptional activity of KLK1 as compared with the A, B, and H alleles in both HEK-293 and IMR-32 cells. However, when the genotype and allele frequencies with or without K allele were analyzed, no statistically significant difference as observed between PD patients and controls. Therefore, the results indicate that although associated with lower transcriptional activity, KLK1 -130G(subscript N) K allele as not associated with the risk of Taiwanese PD. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。