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頁籤選單縮合
題名 | Purification of Trypsin Inhibitor from Sweet Potato by Applying Immobilized Trypsin on Glutaraldehyde Activated Chitosan Beads=利用戊二醛活化的幾丁聚糖擔體固定胰蛋白酶以純化甘薯的胰蛋白酶抑制劑 |
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作者 | 趙坤山; 段國仁; 林銘澤; | 書刊名 | Journal of the Chinese Institute of Chemical Engineers |
卷期 | 37:2 民95.03 |
頁次 | 頁125-129 |
分類號 | 346.78 |
關鍵詞 | 甘薯; 幾丁聚糖; 胰蛋白酶抑制劑; Affinity chromatography; Chitosan beads; Sweet potato; Trypsin immobilization; Trypsin inhibitor; |
語文 | 英文(English) |
中文摘要 | 本研究利用戊二醛活化的幾丁聚糖來固定胰蛋白?作為親和層析管柱的擔體,用來純化甘薯塊根的胰蛋白?抑制劑,研究中探討最適的固定化方法,每公克的幾丁聚糖能固定10單位活性的胰蛋白?。利用此親和層析法僅需一步驟便能從甘薯的粗抽出液中純化56%的胰蛋白?抑制劑,利用SDS-PAGE發現7條可辨識的色帶,推測為胰蛋白?抑制劑,它們的分子量都超過20kDa。 |
英文摘要 | Immobilized trypsin on the glutaraldehyde activated chitosan beads was employed to purify trypsin inhibitor from the tuberous roots of sweet potato by means of affinity chromatography. The optimum conditions for the preparation of immobilized trypsin were investigated, and a maximum trypsin activity of 10 unit/g-beads was achieved in this study. Fify-six percent of the activity of the trypsin inhibitor in the crude extract of sweet potato could be recovered through affinity chromatography. Seven visible bands by SDS-PAGE were suspected to the trypsin inhibitors, and all of the molecular weights were more than 20 kDa. |
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