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題 名 | Osteoblastic Differentiation of Rabbit Mesenchymal Stem Cells Loaded in a Carrier System of Pluronic F127 and Interpore=間葉幹細胞在人工代用骨複合物(Pluronic + Interpore)的骨生成能力評估 |
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作 者 | 黃炤文; 陳文哲; 廖順奎; 楊春勇; 林松樞; 吳基銓; | 書刊名 | 長庚醫學 |
卷 期 | 29:4 民95.07-08 |
頁 次 | 頁363-372 |
分類號 | 410.1644 |
關鍵詞 | 間葉幹細胞; 人工代用骨; Mesenchymal stem cell; Bone substitute; |
語 文 | 英文(English) |
中文摘要 | 背景:理想的人工代用骨必須有骨傳導與骨引導的能力。根據組織工程的原則,間葉幹細胞加入適當的人工代用骨,可以做為自體骨移植的代用品。我們使用interpore做為支架並加入包被在pluronic F-127中的間葉幹細胞,在引導生成的環境中,去測試骨生成能力。 方法:從兔子骨隨中抽取的間葉幹細胞在引導生成的環境中培養。然後我們將細胞在水膠pluronic F-127的協助下,加入支架interpore中。在培養7至14天後,使用Alizarin Red S染色,RT-PCR,鈣濃度,鹼性磷酸酶濃度和掃瞄電子顯微鏡測試骨生成能力。 結果:在7天與14天的培養後,鈣濃度,鹼性磷酸酶活性都增加。Alizarin Red S染色則顯示間葉幹細胞能在細胞/水膠支架系統中存活並分化成骨細胞。RT-PCR則顯示出osteopontin,Cbfa-1。掃瞄電子顯微鏡展示出細胞長在孔洞上。 結論:具有骨傳導性的人工代用骨-interpore,在臨床上使用已有一段時間。在這個研究中,間葉幹細胞可以經由hydrogel-pluronic F-127的協助,而附著在interpore上,並進而達成成骨母細胞的分化。 |
英文摘要 | Background: Ideally, bone tissue engineering products should have the ability of osteoconduction and osteoinduction. According to the tissue engineering principle, mesenchymal stem cells (MSCs) combined with an appropriate scaffold can be used as a bone substitute for bone defects. Here we used Interpore as a scaffold loaded with MSCs mixed in hydrogel (Pluronic F127). In order to demonstrate the osteogenic ability of MSCs in the hydrogel, cell/hydrogel scaffold constructs were cultured in an induction medium to elicit an osteoblastic response. Methods: MSCs aspirated from rabbit bone marrow were cultured in induction medium. MSCs were then loaded into scaffold Interpore with the aid of hydrogel (Pluronic F127). After culture for 7 and 14 days, osteoblastic differentiation ability was tested using Alizarin Red S stain, reverse transcription polymerase chain reaction (RT-PCR), measurement of calcium and alkaline phosphatase levels, and scanning electron microscopy (SEM). Results: Calcium and alkaline phosphatase levels both increased after 7 and 14 days incubation. Alizarin Red S staining revealed MSCs could survive and differentiate to osteoblasts in the cell/hydrogel scaffold. RT-PCR showed mRNA expression of osteopontin and Core binding factor alpha 1 (Cbfa1). SEM revealed growth of osteoblast-like cells on ceramic pores. Conclusions: Osteoconductive bone substitute (Interpore) has been used clinically for a long time. This study showed that MSCs could be held on Interpore with the aid of hydrogel (Pluronic F127) and that they could differentiate to osteoblasts. |
本系統中英文摘要資訊取自各篇刊載內容。