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題名 | 酸鹼、熱處理及儲藏條件對微膠囊化牛初乳與乳清中IgG 安定性之影響=Effects of pH, Thermal Processing, and Storage Conditions on the Stability of Immunoglobulin G (IgG) in Microencapsulated Bovine Colostrums and Whey |
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作者 | 陳志瑋; 沈子偉; 江淑華; 王秀育; 張基郁; Chen, Chih-wei; Shen, Tzu-wei; Chiang, Shu-hua; Wang, Shiu-yu; Chang, Chi-yue; |
期刊 | 科學與工程技術期刊 |
出版日期 | 20101200 |
卷期 | 6:4 2010.12[民99.12] |
頁次 | 頁59-66 |
分類號 | 463 |
語文 | chi |
關鍵詞 | 牛初乳; β-環狀糊精; 幾丁聚醣; 阿拉伯膠; 微膠囊化; Bovine colostrums; β-cyclodextrin; β-CD; Chitosan; Gum arabic; Microencapsulation; |
中文摘要 | 本研究以取自母牛分娩後第2、3、4 天分泌且等體積混合的乳汁作為材料,以阿拉伯膠、β-環狀糊精或幾丁聚醣之10% 水溶液,分別依1:4 體積比和牛初乳或乳清混合,以冷凍及噴霧乾燥法進行微膠囊化,並探討pH 值、熱處理及儲藏時間對微膠囊化初乳與乳清中IgG 安定性之影響。結果顯示,在酸鹼安定性方面,未微膠囊化之初乳在pH 7~8 有較高之IgG 活性,經微膠囊化者則以阿拉伯膠所得者在pH 7~8 時有較高之IgG 活性。在熱安定性方面,阿拉伯膠和β-環狀糊精對於初乳或乳清之IgG 均具有保護作用。在儲藏條件方面,儲藏溫度與包裝材質對於初乳或乳清之IgG 殘存活性有顯著影響,在4°C 儲存60 天後之IgG 活性較室溫高,以鋁袋包裝儲存者高於以透明塑膠袋包裝者,以阿拉伯膠微膠囊化之初乳在4°C 儲存60 天後之IgG 殘存活性顯著高於未微膠囊化者。 |
英文摘要 | The bovine colostrums collected from the second, third, and fourth days postpartum were mixed with an equal volume and used as the raw materials. A 10% aqueous solution of gum arabic, β-cyclodextrin or chitosan was added in an equal volume of colostrums or whey, and microencapsulated through freeze-drying and spray-drying methods. The effects of pH, thermal processing, and storage conditions on the stability of the immunoglobulin G (IgG) in microencapsulated colostrums and whey were investigated. Results showed that non-microencapsulated colostrums were found to have higher IgG activity at pH 7~8 than at other pH values. The microencapsulated colostrums with gum arabic were found to have higher IgG residual activity at a pH 7~8. For thermal stability, gum arabic and β-cyclodextrin (β-CD) were found to have a protective effect on IgG activity of colostrums and whey. For storage conditions, storage temperature and packaging materials were found to have a significant impact on residual IgG activity of colostrums and whey. After being stored for 60 days at 4 °C, the IgG activity was found to be higher than those stored at room temperature were. As for packaging materials, aluminum bag-packed colostrums and whey were found to have higher IgG activity than transparent plastic bag-packed colostrums. After storage for 60 days at 4 °C, the microencapsulated colostrums with gum arabic were found to have significantly higher IgG residual activity than the non-microencapsulated colostrums. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。