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題名 | 利用高效液相層析法分析化粧品中Estradiol、Ethinyl Estradiol及Estrone等成分=Simultaneous Determination of Estradiol, Ethinyl Estradiol and Estrone in Cosmetics by HPLC |
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作者 | 陳信豪; 黃守潔; 陳玉盆; 闕麗卿; 施養志; Chen, Shin-hao; Huang, Shou-chieh; Chen, Yu-pen; Chiueh, Lih-ching; Shih, Daniel Yang-chih; |
期刊 | 食品藥物研究年報 |
出版日期 | 20121100 |
卷期 | 3 2012.11[民101.11] |
頁次 | 頁252-256 |
分類號 | 412.36 |
語文 | chi |
關鍵詞 | 高效液相層析法; Estradiol; Ethinyl estradiol; Estrone; HPLC; |
中文摘要 | 本研究係利用高效液相層析配合光二極體陣列檢出器,同時分析化粧品中Estradiol、Ethinyl estradiol及Estrone成分。檢體先經甲醇溶解定容過濾後,以高效液相層析儀進行分析。使用之層析管柱為Lichros-pher RP-18(管徑4.0 mm × 250 mm,5 μm);移動相為去離子水和乙腈比例為50:50(v/v);流速為0.8 mL/min;檢測波長為280 nm。三種成分標準曲線之線性範圍為1.0-20.0 μg/mL,線性回歸之判定係數(r2)介於0.9974-1.0000之間,同日內及異日間試驗之相對標準偏差均小於9.1%。添加不同濃度之標準品於空白面霜中,回收率介於86.1-117.6%,相對標準偏差均小於2.2%。最低定量濃度則均為5.0 ppm。應用本研究所建立之方法檢測市售化粧品11件,除1件檢體檢出estradiol外,其餘均未檢出。 |
英文摘要 | A high performance liquid chromatographic method was developed for simultaneous determination of Estradiol, Ethinyl estradiol and Estrone in cosmetics, using Lichrospher RP-18 (I.D. 4.0 mm × 25 cm, 5 μm) column. In order to investigate the quality of marketed cosmetics, 11 samples were randomly collected by local health bureaus in Taiwan area during the year of 100. A mixture of water and methanol (50 : 50, v/v) was used as mobile phase. Flow rate was 0.8 mL/min and the chromatography was monitored by absorbance at 280 nm wavelength. The related coefficients R2, of regression equations of the three calibration curve were 0.9974-1.0000. The relative standard deviations (RSD) of the three estrogen ingredients for intraday and interday analysis of relative standard deviation (RSD) were less than 9.1%. The average recoveries of these three ingredients spiked in sample ranged from 86.1 to 117.6%. The RSD’s of average recoveries were less than 2.2%. The limits of quantitation in cosmetics were 0.5 μg/mL for Estradiol, Ethinyl estradiol and Estrone. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。