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題名 | 保存條件對山羊與牛血液樣品孕酮及若干生化值之影響=Influence of Storage Conditions on Progesterone and Blood Analytes Concentrations in Blood Samples from Goats and Cows |
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作者 | 邱智賢; 楊健仁; 林仁壽; 鍾德憲; 吳兩新; Chiu, Chih-hsien; Yang, Chien-jen; Lin, Jen-hsou; Jong, De-shien; Wu, Leang-shin; |
期刊 | 動物園學報 |
出版日期 | 20051200 |
卷期 | 17 民94.12 |
頁次 | 頁1-12 |
分類號 | 383.9 |
語文 | chi |
關鍵詞 | 牛; 山羊; 孕酮; 血液生化學; Cow; Goat; Progesterone; Blood biochemistry; |
中文摘要 | 動物血液於採血後至離心前的檢體保存條件,會影響隨後血漿或血清中之生化成分。本試驗以山羊及乳牛為試驗動物,血樣採集後於室溫下保存0-48小時,經分析其孕酮(progesterone)及若干血液生化值濃度。結果顯示牛隻血液檢體車孕酮含量,在室溫下隨保存時間的延長而顯著下降,尤以前6小時之血樣下降最為顯著;然而山羊之血液檢體則不受影響。暗示血液檢體中孕酮含量會因血球的存在而下降,且代謝孕酮之能力可能具有動物種別差異。另檢測山羊與乳牛血漿與血清中十項血液生化值,結果顯示葡萄糖含量隨採血後至離心時間的延長而顯降低,尤其是乢牛血液檢體,於室溫保存6小時後,無論血漿或血清葡萄糖含量均降至10 mgdL以下;而天冬胺酸轉胺酶含量則隨保存時間的延長升高。而其餘血液生化值包括總蛋白質、白蛋白、尿素氮、肌酸酐、總膽紅素、丙胺酸轉胺酶、乳酸鹽報氫酶及鹼性磷酸酶濃度,則無顯著變化。綜合本研究結果顯示,採血後至分離血球前的血樣,若保存於室溫下會影響血漿或血清中孕酮、葡萄糖與天冬胺酸轉胺酶濃度。 |
英文摘要 | Storage conditions have always played a crucial role in disparity of clinical findings. This storage conditions have always played a crucial role in disparity of clinical findings. This experiment was undertaken to analyze the influence of storage conditions on progesterone (P4) and ten different kinds of blood analytes concentrations in blood obtained from goats and cows. The whole blood was stored at room temperature of 0 to 48 hours prior to centrifugation. The enzyme immunoassay results for the concentration of P4 levels in plasma and serum from cows decreased significantly (P<0.05) with increasing storage time at room temperature. The onset of decline in the P4 level began fast as evident from the sharp decline at six hours compared to levels in the fresh sample of the same specimen. Interestingly, no such significant decline pattern was noticed in the goat serum and plasma samples assay. Analyzing the samples for blood biochemistry revealed that glucose concentration in both goat and cow decreased significantly (P<0.05) compared to the fresh specimen over the room temperature storage duration. Of the other blood anlaytes assayed, aspirate transaminase (AST) showed increased level with storage duration, while for total preotein, albumin, urine nitrogen, creatinine, total bilirubin, alanine transaminase, lactate dehydrogenase, alkaline phosphatase there were no noteworthy changes. This experiment implied that the presence of steroids degrading components in whole blood antributed by the cell components. The same was however, not true for goats, possibly indicating a species variation. Of the analytes chosen, glucose showed decrease with storage time, while AST showed increase. The possible role of these analytes in decrease of P4 level provides interesting avenue for further research. It is also interesting to find out as to why; the difference in goat and cow exists as evident from our observation. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。