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| 題 名 | Bio-Degradation of Pristane in Mixed Alkanes by a Mixed Culture Containing Nocardia corynebacterioides=以混合菌分解油品中之異十九烷時Nocardia corynebacterioides扮演之角色 |
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| 作 者 | 阮若屈; 于大鈞; | 書刊名 | Journal of the Chinese Institute of Chemical Engineers |
| 卷 期 | 35:6 2004.11[民93.11] |
| 頁 次 | 頁651-658 |
| 分類號 | 460.024 |
| 關鍵詞 | 混合菌; 異十九烷; Diesel; n-Pentadecane; Pristane; Nocardia corynebacterioides; Rhodococcus globerulus; |
| 語 文 | 英文(English) |
| 中文摘要 | 我們在南臺灣一處受柴油污染的場址中,篩選到一株可以分解直烷與支烷粉的細菌,經確認後發現是Nocardia corynebacterioides。無機的氮、磷養分添加,均無法促進此菌的生長與其對烷類的分解,但有機養分如酵母萃取物則可以刺激此菌的生長,因而增加烷類降解。此菌特別的是其具有相當好的異十九烷降宗解能力,唯此能力嚴重的受到直烷類的抑制,在同一受污染的場址中,我們也分離出一株降解直烷類效果很好的菌株,Rhodococcus globerulus。實驗發現加入此菌可幫助Nocardia corynebacterioides在直烷與支烷的混合統統中降解異十九號。在此篇文章中,我們分別探討此兩菌株在直十五烷、異十九烷及混合碳減中的分解能力,對此兩菌的合作情形也進行了解。我們發現以光學濁度1:1的殖入比來降解直十五烷與異十九烷1:1的混合物,其降解的效果最佳。以上結果指出有機養分的添加,並針對污染場址之碳氫化合物組成來控制菌相比例,將有助於柴油污染場址之復育。 |
| 英文摘要 | A Gram-positive bacterium identified as Nocardia corynebacterioides L29-2 was isolated from a diesel oil contaminated site in southern Taiwan. The bacterium was found able to degrade both straight and branched alkanes in diesel oil. Inorganic nutrients were not able to enhance the growth of the cells but the cell growth and hydrocarbon biodegradation could be accelerated by adding yeast extract. It was found that this bacterium was able to degrade pristine at high efficiency. However, the ability of pristine biodegradation was severely inhibited by the presence of normal alkanes. The diesel oil biodegradation was significantly improved by introducing another bacterium, Rhodococcus globerulus H425-2, found in the same contaminated site. Rhodococcus globerulus H425-2 was found to degrade normal alkanes at a much higher rate then Nocardia corynebacterioides L29-2. In this study, the biodergradation of pristine and n-pentadecane in single substrate and mixed substrate experiments were compared. The cooperation of Nocardia corynebacterioides L29-2 and Rhodococcus globerulus H425-2 was also under investigation. It appeared that the 1:1 ratio was close to the optimum inoculation ratio of these two species for degrading the mixture of pristine and n-penadecane at 1:1 ratio. The results indicate that the addition of organic nutrients and the proper control of the population ration according to the alkane composition in the contaminated site are crucial for effective bioremediation of diesel spill. |
本系統中英文摘要資訊取自各篇刊載內容。