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題名 | 不良遺傳型頻率與混合樣本數對 DNA 遺傳檢測的經濟效益影響=Influence of Undesired Genotype Frequency and Number of Samples Mixed on Economic Efficiency of Genetic Testing |
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作者 | 黃鈺嘉; 林德育; 陳若菁; 楊德威; 張秀鑾; Huang, Yu-chia; Lin, Der-yuh; Chen, Jo-ching; Yang, Tei-wei; Chang, Hsiu-luan; |
期刊 | 畜產研究 |
出版日期 | 20000300 |
卷期 | 33:1 2000.03[民89.03] |
頁次 | 頁48-53 |
分類號 | 437.21 |
語文 | chi |
關鍵詞 | 混合DNA; 混合樣本; 遺傳檢測; Mixed samples; DNA mixes; Genetic testing; |
中文摘要 | 以雜合型及正常型牛淋巴球黏力缺失症母牛(BL與TL)的全血或DNA進行1:1、1:3、1:4至1:9的混合後,再以混合的樣本進行後續的遺傳檢測工作,如DNA的聚合?連鎖反應與限制?切割等。結果顯示即使是1:9的混合仍可測出雜合型(BL)的存在。但是隨著混合樣本數的增口,膠片上雜合型特有的條帶就會漸淡,在5隻樣本混合時(1:4)呈相的效果仍不錯。經濟效益模擬分析:假設共有1000隻待檢測動物,依不良遺傳型的頻率變化與不同的混合樣本數對DNA遺傳檢測所需的總次數進行估算,只要混合樣本中測出有雜合型者,則需回溯重測個別原未混合的樣本。估算結果顯示:不良遺傳型的頻率在0.1%之下時,只要能正確的檢測出帶有雜合型的混合樣本,則越多的樣本混合應越有經濟效益,但是隨著不良遺傳型的頻率增加到10%,則混合樣本的效益就不存在。綜合所述,以現階段的技術而言,混合樣本之DNA遺傳檢測可應用於經濟價值較低且不良遺傳型頻率很低(反之或接近100%時)的遺傳篩選應用。 |
英文摘要 | Samples of whole blood of different genotypes of Bovine Leukocyte Adhesion Deficiency, BL and TL (carrier and normal), were mixed from 1:1, 1:3 1:4 to 1:9 and then had the DNA extracted, followed by genotyping procedures. In comparison, the mixed samples collected from extracted DNA from individual animal were mixed in the same ratio. Both restricted enzyme PCR products showed that from up to 1:9 mixed samples the existence of carrier (BL) genotype could be detected. However, the increase of the number of samples also decreased the blackness of the band of the image, and the five samples mixed, in the 1:4, could generate acceptable image for visual judgement. Simulation study of economic efficiency of DNA test was based on frequencies of undesired genotypes of 1,000 animals and numbers of samples mixed. If the mixed sample was detected and contained undesired genotypes, a redo test would need to be performed for each unmixed sample. As the undesired genotypes were under 0.1%, the more samples mixed the less tests were required. However, if the undesired genotypes were more than 10%, mixed samples showed only little benefit over unmixed test. Mixed DNA test could be applied in screening undesired genotypes for animals with less economic values especially when the undesired genotypes frequency was low. |
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