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頁籤選單縮合
題名 | Strategies for Diagnosing HIV-1 Infection in Atypical Western Blots=人類後天免疫不全病毒感染之血清學診斷 |
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作者 | 陳茂源; 李光倫; 洪健清; 莊哲彥; 周民治; Chen, Mao-yuan; Lee, Kuang-lun; Hung, Chien-ching; Chuang, Che-yen; Chou, Min-ji; |
期刊 | 中華民國微生物及免疫學雜誌 |
出版日期 | 19970800 |
卷期 | 30:3 1997.08[民86.08] |
頁次 | 頁144-135 |
分類號 | 414.84 |
語文 | eng |
關鍵詞 | 後天免疫不全病毒感染; 血清學診斷; Human immunodeficiency virus type 1; HIV-1; Indeterminate western blot; WB; HIV-1 vpu; Seroconversion; RT-PCR; |
中文摘要 | 以第三代免疫酵素分析法偵測後天免疫不全病毒感染其特異性已與西方墨點法相差無幾。然而西方墨點法仍因其連續性的變化為血清由陰性轉陽性以及臨床病情進行的重要判斷依據而無可取代。不過西方墨點之判讀標準有多套不同版本,且偶而出現困難判讀之結果。 病毒感染之初期及末期為較易出現不典型而無法判讀(indeterminate)之西方墨點結果。本報告中針對兩例在西方墨點上只有gp160陽性之罕見個案示範在困難判別情形下,可以聚合□連鎖反應放大HIV-1 vpu或密切追蹤一段期間內之系列血清,即可得到正確之診。 本研究中另外就陽性而非典型之西方墨點測試第二型免疫不全病毒之存在証據,結果並未發現陽性之血清。 |
英文摘要 | The Western blot (WB) has long been used to confirm positive ELISAs for diagnosing HIV-1 infections. However, some WB patterns may result in "indeterminate" " or controversial reports thus impeding early diagnoses or accurate diagnoses. The interpretation of HIV-1 WB has no "gold standard" criterion. Incomplete antibody profiles on WB strips can be interpreted as positive or indeterminate according to different criteria. Thc possibility of HIV-2 infection was further checked in these serum samples. However, no reactivity to synthetic peptide of HIV-2 gp36 had been found. Serial WB analyses arc important for attaining early diagnoses of HIV-1 infections as well as for evaluating clinical stages. Temporal changes on WB patterns of serial serum samples provide the evidence of seroconversion in individuals with risk behaviours and indeterminate WB. In late stage of HIV-1 infection, the reactivity to gag, pol and env antigen groups may decrease and result in indeterminate WB. We propose to diagnose HIV-1 Infection and to differentiate the infection of HIV-1 from HIV-2 in these cases by using nested polymerase chain reaction (PCR) to demonstrate the presence of HIV-1 specific vpu gene. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。