頁籤選單縮合
題名 | Apoptotic Phenomenon of Immune Effector Cells in Autoimmune NZB x NZW F1 Mice=自體免疫傾向小鼠的免疫細胞之凋亡現象 |
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作者 | 陳嫈諭; 孫昭玲; 吳文勉; 江伯倫; Chen, Ying-yu; Suen, Jau-ling; Wu, Wen-mein; Chiang, Bor-luen; |
期刊 | 微免與感染雜誌 |
出版日期 | 19990600 |
卷期 | 32:2 1999.06[民88.06] |
頁次 | 頁83-89 |
分類號 | 414.82 |
語文 | eng |
關鍵詞 | 自體免疫; 免疫細胞; 細胞凋亡; Systemic lupus erythematosus; Spleen cells; Peritoneal cells; DEX-induced apoptosis; |
中文摘要 | 細胞凋亡(Apoptosis)的過程和各種組織的發展有著密切的關係,尤其是對免疫系統而言,更扮演著極重要的角色。自體免疫性的未成熟的T和B淋巴球,當其第一次遇到自體抗原時,會引發細胞凋亡的進行。為了進一步探討細胞凋亡在自體免疫疾病病理機轉上所扮演的角色,因此我們以一種會類似人類紅斑性狼瘡的小鼠NZB/W F1為模式,以正常的小鼠BALB/c作為對照組,分離出脾臟及腹腔細胞,藉由DNA染色的技術,比較細胞凋亡的情形。其結果如下:1)新鮮分離的BALB/c及NZB/W F1小鼠脾臟細胞,經DNA染色後,藉由流式細胞分析儀定量分析,均顯示極少比例的細胞凋亡;且隨著年齡的增加,而有統計上的顯著增加( p<0.001);2) NZB/W F1小鼠的脾臟細胞的自發性的細胞凋亡比例較 BALB/c 的高(p<0.05);3 ) Dexamethasone(DEX)處理後的NZB/W F1小鼠的脾臟細胞凋亡的比例較 BALB/c 小鼠為低;4)新鮮分離的BALB/c及NZB/W F1小鼠的腹腔細胞,均顯示極少比例的細胞凋亡;且隨著年齡的增加,而有統計上的顯著增加(p<0.05);5)DEX處理後的BALB/c 小鼠的腹腔細胞凋亡的比例隨著年齡的增加而顯著的增加;而 NZB/W F1小鼠的則否。由我們的實驗結果可清楚看出脾臟細胞和腹腔細胞的凋亡反應十分不同;而且,自體免疫傾向的小鼠和正常的小鼠的免疫細胞的凋亡反應也不相同。 |
英文摘要 | Programmed cell death (apoptosis) is a process involved in the development of various tissues and suggested to be critical in the elimination of self-reactive immature T and B lymphocytes when they first encounter self antigens. To investigate further the role of apoptosis in the pathogenesis of autoimmune disease, the apoptotic phenomena of spleen cells and peritoneal cells of NZB/W F1 mice or nonautoimmune BALB/c mice wewe studied. Spleen cells and peritoneal cells were isolated and analyzed for their apoptotic phenomenon with the method of DNA staining. The data showed: 1) freshly isolated-spleen cells from BALB/c and NZB/W F1 mice showed little apoptosis as assessed by quantitative DNA flow cytometry, and had a significant increase with age (p<0.001); 2) and data also showed that spleen cells of NZB/W F1 have higher spontaneous apoptotic rate than those of BALB/c mice (p<0.05); 3) the apoptotic rate of dexamethasone (DEX)-treated spleen cells was lower in NZB/W F1 mice compared to that of nonautoimmune mice; 4) peritoneal cells showed little apoptosis when cells were freeshly isolated and also had a striking increase with age (p<0.05); 5) the apoptotic rate of DEX-treated peritoneal cells increased dramatically with age in BALB/c (p<0.05), but not in NZB/W F1 mice. Our data showed quite clearly that splenic cells are much different from peritoneal cells according to their apoptotic responses. Furthermore, the apoptotic phenomenon of immune effector cells was also different between those of autoimmune and normal mice. |
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