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題 名 | 應用膨脹床離子交換層析技術直接回收穀胱甘呔轉移酵素=Application of Expanded Bed Ion Exchange Chromatographic Technology for Direct Recovery of Glutathione-S-transferase |
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作 者 | 陳泰宏; 葉昭賢; 陳秀美; 張煜光; | 書刊名 | 明志學報 |
卷 期 | 34:1 2002.06[民91.06] |
頁 次 | 頁65-74 |
分類號 | 460.025 |
關鍵詞 | 大腸桿菌BL 21; 穀胱甘呔轉移酵素; 膨脹床離子交換層析法; E. coli BL21; Glutathione-S-transferase; Expanded bed ion exchange chromatography; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究將帶有穀胱甘呔轉移酵素基因之質體pGEX-5X-2,送入大腸桿菌E.coli BL21中表現,以生產穀胱甘呔轉移酵素。應用膨脹床吸附技術,從未澄清破碎菌液中,直接回收目標酵素。結果顯示,選用2.0M氯化鈉為沖提溶液,酵素回收率達62%,而純度提昇約二倍左右。若以GST酵素純化程序的回收率與純度提昇而言,離子交換層析法較固定化金屬親和性層析法更適合於大規模的直接回收程序。 |
英文摘要 | In this study, the plasmid pGEX-5X-2 carrying glutathione-S-transferase GST gene was expressed and transformed into E. coli BL21 used to produce GST enzyme. Expanded bed adsorption technology was applied to direct recovery of target enzyme from unclarified E. coli homogenates. The results showed that the recovery of GST enzyme by using 2.0 M NaCl as an eluent was 62%. The purification factor was approximately two-fold. Compared with yields and folds of purification, ion exchange chromatography is more suitable for GST enzyme purification than immobilized metal affinity chromatography in a large-scale direct recovery process. |
本系統中英文摘要資訊取自各篇刊載內容。